玉米抗大斑病相关基因片段的分离.pdfVIP

第24卷第4期 阿 方学院学亦(自然科学版) Vo1．24 No．4 2008年8月 Journal of Hebei North University(Natural Science Edition) Aug．2008 玉米抗大斑病相关基因片段的分离 郝 敏 ，韩建民 ，曹柳青。 (1．河北北方学院医学技术学院，河北 张家口075000； 2 河北农业大学真菌毒素实验室，河北保定071001；3．保定学院，河北保定071001) 摘要：目的 利用mRNA差异显示技术有效地比较组织或细胞问的差异表达基因．方法 以玉米自交系 B37Htl与玉米大斑病菌0号及1号小种构成非亲和性互作与亲和性互作体系，利用mRNA差异显示技术对接 种玉米大斑病菌后玉米叶片早期表达基因进行分析，寻找玉米抗大斑病相关基因表达片段．结果 提取玉米总 RNA；经引物筛选．获得8条随机引物；经聚丙烯酰胺凝胶电泳和银染检测，获得差异片段62条．结论 利 用mRNA差异显示技术能够获得玉米抗大斑病相关基因片段． 关键词：抗病基因；玉米大斑病；mRNA差异显示 中图分类号：S 435．13 文献标识码：A 文章编号：1673—1492(2008)04—0032—04 Separation of Corn Gene Fragments Related to Resistance to Setosphaeria turcica HAO Min ，HAN Jian：min。，CAO Liu-qing。 (1．Medical Technology College，Hebei North University，Zhangjiakou 075000，Hebei，China； 2．Mycotoxin Lab of Agricultural University of Hebei，Baoding 071001，Hebei，China； 3．Baoding College，Baoding 071001，Hebei，China) Abstract：Objective To effectively compare the differential expressed gene fragments with normal tissue or cells by mRNA differential display reverse transcription polymerase chain reaction (DDRT- PCR)．Methods The gene fragments expressed differently in the corn leaves were studied and analysed both in incompatible combination (B3 7 Htl inoculated with race 0 of Setosphaeria turcica)and in compati— ble combination(B37Htl inoculated with race 1 of Setosphaeria turcica)by mRNA differential display PCR．The amplification products were compared under the condition of background with one accord to ob— tain the gene fragments related to resistance to Setosphaeria turcia．Results 6 2 gene fragments expressed differently were obtained by PAGE． Key words：disease-resistant gene；northern corn leaf blight；DDRT—PCR