轻度认知功能障碍患者淋巴细胞糖原合成酶激酶-3活性的变化.pdfVIP

· 94· J Clin Neurol，April 2008，Vo1．21，No．2 · 论著 · 轻度认知功能障碍患者淋巴细胞糖原合成酶 激酶一3活性的变化 李亮，王建华，曲忠森 【摘要】 目的 探讨轻度认知功能障碍(MCI)患者淋巴细胞糖原合成酶激酶-3(GSK-3)活性的变化。 方法 应用免疫印迹法检测不同病程(1年，2年，3年)MCI患者及正常老年人(正常对照组)外周血淋巴细 胞GSK-3B Ser9位点的磷酸化水平及总GSK-313水平。结果 1年，2年及3年MCI组淋巴细胞GSK-313 Ser9 位点磷酸化水平比正常对照组显著降低(均P0．01)；总GSK．3B水平与正常对照组相比差异无统计学意义； MCI组间淋巴细胞GSK．3B Ser9位点磷酸化水平及总GSK-313水平差异无统计学意义(均P0．05)。结论 MCI患者淋巴细胞GSK-3的活性升高，对MCI的诊断可能有参考价值。 【关键词】 轻度认知功能障碍；淋巴细胞；糖原合成酶激酶．3 【中图分类号】R749．1 【文献标识码】A 【文章编号】1004-1648(2008)02-0094-03 Change of glycogen synthase kinase-3 activity in Iymphocytes in pafien~with mild cognition impairment L／Liang，WANG Jian—hua，QU Zhong—sen．Department of Neurology，Jiao．zhou Central Hospital of Qingdao， Jiaozhou 266300． ina Abstract：Objective To explore the change of glycogen synthase kinase-3(GSK-3)activity in lymphocytes in patients with mild cognition impairment(MCI)．Methods The phosphorylation of GSK-3 13 at Ser 9 site and total level of GSK-3 13 in pefipherM blood were measured by Western—blot in normal control group，one-year MCI group， two—year MCI group and three-year MCI group．Results The phosphorylation of GSK一3B at Ser 9 site were lower in 1一year MCI group，2-year MCI group and 3-year MCI group，compared with normal control group(all P0．01)． There were not significant difference in total level of GSK一3 B among MCI group and normal control group and in phosphorylation of GSK-3B at Ser 9 site and total level of GSK-3B in MCI group(all P0．05)．Conclusion The activity of GSK-3 in lymphocytes in MCI patients is raised，which implies a subsidiary value of MCI diagnosis． Key words：mild cognition impairment； lymphocyte； glycogen synthase kinase-3 轻度认知功能障碍(MCI)被认为是一种介于老 化，尚未见报道。本研究检测不同病程MCI患者外 年记忆减退和痴呆之间的过渡状态，研究…显示MCI 周血淋巴细胞GSK一3B Ser9位点磷酸化及总GSK一3p 患者1年内约有10％ 一15％、