泰素抑制涎腺腺样囊性癌远处转移的机制研究.pdfVIP

天津医药2008年4月第36卷第4期 283 泰素抑制涎腺腺样囊性癌远处转移的机制研究 刘 浩 俞光岩 摘要 目的：观察泰素 (Taxo1)对涎腺腺样囊性癌细胞远处转移的作用及其对肺转移灶血管内皮生长 子 (VEGF)、基质金属蛋白酶(MMP_9)表达的影响。方法：用涎腺腺样囊性癌肺高转移细胞株(ACC—M)裸鼠肺转移模型观 察泰素体内抗转移效果，免疫组化法检测转移灶肿瘤细胞VEGF和MMP一9的表达。结果：对照组与Taxol治疗组相 比，2组之间瘤结节数差异有统计学意义(P0．05)；Taxol治疗组YEGF及MMP一9表达明显弱于对照组(P0．05 o 结论：泰素对涎腺腺样囊性癌肺转移有较好的抑制效果，这可能与其抑制血管生成及基质金属蛋白酶表达有关。 关键词 涎腺肿瘤 囊腺癌 肿瘤转移 紫杉酚 细胞外基质 An Study on Taxol against the Metastasis of Salivary Adenoid Cystic Carcinoma LIU Hao．YU Guangyan Department ofStomatology，TianjinRenminHospital，Tianjin 300121，China Abstract Objective：To study the effect of taxol on experimental metastasis of ACC—M and the expression of VEGF and MMP一9 in vivo．Methods：The antimetastasis effect of taxol was studied in vivo experiments using nude mice model of pulmonary metastasis with ACC—M cell line．Th e expression of VEGF and MMP一9 of the lung metastases were also observed．Results：The numbem of pulmonary metastases was significantly reduced in treatment group(P0．05)．The levels of VEGF and MMP-9 positive expression were decreased in treatment group (P0．05)．Conclusion：Taxol has a strong antimetastasis effect on experimental metastasis of ACC-M．Th is may be relative to the inhibiting of the expression of VEGF and MMP-9 and vascular growth． Key wO s salivary gland neoplasms cystadenocarcinoma neoplasm metastasis paclitaxel extracellular matrix 涎腺腺样囊性癌 (salivary adenoid cystic carci— 1．2 方法 noma，SACC)是口腔颌面部最常见的涎腺恶性肿瘤 1．2．1 体内实验 ACC—M细胞常规体外培养，将处于指数 之一，它的最大特征是侵袭力强，易发生远处转移， 生长期的细胞经0．125％胰酶消化，吹打成细胞悬液，细胞经 1 000 r／min离心5 min，弃去上清，加入Hank S液吹打后冉离 尤其以肺部转移最为常见，常用的化疗药物控制腺 心，重复3次，用无血清RPMI 1640培养液稀释细胞浓度为 样囊性