鹅3-羟基-3-甲基戊二酸单酰辅酶A还原酶（HMGR）基因内含子5的SNP及其与鹅重要经济性状的关联性.pdfVIP

农业生物技术学报 Journal ofAgricultural Biotechnology 2007，1 5 (6)：936-94 1 · 研究论文· 鹅3一羟基一3一甲基戊二酸单酰辅酶A还原酶(HMGR)基因内 含子5的SNP及其与鹅重要经济性状的关联性 木 梁 忠 一，陈宏权 · 料，黄华云 ，查丽莎 ，陈 华 (1．安徽农业大学动物科技学院，合肥230036；2．安徽农业大学生命科学学院，合肥230036) 摘要：屠宰测定了l16只皖西白鹅(Anseranser)杂种一代鹅(母本为四JiI白鹅(A．anser))的胴体性状、羽绒性状和肉质性 状，克隆了鹅3．羟基一3一甲基戊二酸单酰辅酶A还原酶(HMGR)基因内含子5，PCR．SSCP检测其SNP，并分析了SNP与鹅重 要经济性状之间的关联性。结果显示，鹅HMGR基因内含子5长704 bp，与鸡的同源性44．0％；PCR．SSCP检测发现，内含子5 存在1个SNP位点，带有l对等位基因A和B，其基因频率分别为0．7716和0．2284。分析不同基因型与性状的关联性表明， AA型对提高鹅胸肌重和腺胃重(P0．05)，增加胸肌纤维直径和羽绒的千朵重(P0．01)，以及降低胸肌45pH和绒系水率(P 0．05)具有显著的遗传效应；AB型的遗传效应则与AA型相反。 关键词：鹅；3一羟基一3一甲基戊二酸单酰辅酶A还原酶(HMGR)基因；SNP；经济性状 中图分类号：S188 文献标识码：A 文章编号：1006一l304(2007)06—0936．06 SNP in Intron 5 of 3一hydroxy一3一methylglutaryl Coenzyme A Reductase(HMGR) Gene and Its Genetic Effects on Important Economic Trait in Goose LIANG Zhong 1．2 CHEN Hong—quan · 料，HUANG Hua—yun ，ZHA Li—sha ，CHEN Hua rJ．CollegeofAnimalScience andTechnology,AnhuiAgriculturalUniversity,Hefei230036,China； 2．College ofBiologyScience,AnhuiAgricultural University,Hefei23003~China) Abstract：The intron 5 ofthe 3-hydroxy一3一methylglutaryl coenzyme A reductase(HMGR)gene was cloned and its SNP was test— ed by PCR—SSCP in Wanxi White goose(wW)(Anser anser)．The carcass trait．meat quality trait and feather—down trait of 1 1 6 geese from wW male×Sichuan Wh ite goose(A．anseF)female were mensurated and analyzed by association with SNP．The resulu showed that the sequence length of intron 5 of HMGR gene in goose was 704 bp。and the homology between goose and chicken was 44．0％．A SNP site was tested in the intron 5 by PCR—SSCP，and frequencies ofallele A and B at the SNP site were 0．7716 and 0．2284。respec— tively．The effects of genotype AA on increasing chest muscle weight，gland stomach weight(P0．05)，fiber diame