基于核酸适体和胶体金技术构建凝血酶的dna 逻辑门 - 生命科学研究.pdf

19 卷 3 期 生命科学研究 灾燥造援员9 晕燥援3 圆园15 年6 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Jun. 圆园15 基于核酸适体和胶体金技术构建 凝血酶的DNA 逻辑门 曹青锋袁闫舒雅袁龙佳宝袁孟祥贤* （湖南大学生 学院，中国湖南 长沙410082 ） 摘 要 DNA 逻辑门在分子计算机和分子水平上的计算技术中具有重要作用遥 我们通过设计核酸适体与凝血 酶特异结合导致胶体金团聚的方法袁构建了凝血酶的DNA 逻辑门遥 凝血酶作为输入信号与其核酸适体结合袁 将监测探针上的胶体金结合位点释放袁两个核酸功能化修饰的胶体金同时结合到监测探针上袁引起胶体金团 聚袁实现信号输出遥 根据这个设计原理袁成功构建了蛋白质凝血酶的YES 逻辑门遥 关键词 DNA 逻辑门曰凝血酶曰胶体金曰核酸适体 中图分类号 Q955 文献标识码 A 文章编号 1007-7847(2015)03-0210-03 DNA Logic Gate for Thrombin Based on Aptamer and Gold Nanoparticle Technology CAO Qing-feng, YAN Shu-ya, LONG Jia-bao, MENG Xiang-xian* (College of Biology ，H unan Univers ity, Changsha 410082, H unan, China) Abstract: DNA logic gate plays an important role in the molecular computer and a molecular level of com puter technology. Based on the binding of the aptamer and thrombin and assemble of functionalized gold nanoparticles, a DNA logic gate was constructed. When added thrombin as the input, the binding of throm bin and its aptamer results to release the site of the gold nanoparticle site in a detection probe, in which two gold nanoparticles hybridizes with the probe and causes the assemble of gold nanoparticles and realizes the output signal of logic gate. According to the design principle, the YES logic gate for protein thrombin was successfully constructed. Key words: DNA logic gate; thrombin; gold nanoparticle; aptamer （L ife Sc ience Research ，20 15，19（3 ）：2 10耀212） 以核酸为基础的分子计算机或者分子水平上 汪尔康院士利用核酸置换技术和G 四聚体组装 的信息处理有望代替传统的以硅作为原 的计算 技术构建逻