功能基因组学 4_功能基因组学.pptVIP

Marker Genome Epi-genome Classification Cluster Self-defined Summary Gene expression; Gene module; SNP; CNV; TFBS; DHS; DNA methylation Chromatin modification . . . Unsupervised Altered distance Binary values . . . Survival * * MicroRNA Signature Predicts Survival and Relapse in Lung Cancer Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer LncRNA profile study reveals a three-lncRNA signature associated with the survival of patients with oesophageal squamous cell carcinoma Coexpression Network Analysis Identifies Transcriptional Modules Related to Proastrocytic Differentiation and Sprouty Signaling in Glioma Gliomas are primary brain tumors with high mortality and heterogeneous biology that is insufficiently understood. In this study, we performed a systematic analysis of the intrinsic organization of complex glioma transcriptome to gain deeper knowledge of the tumor biology. Gene coexpression relationships were explored in 790 glioma samples from 5 published patient cohorts treated at different institutions. The primary analysis was focused on the Gravendeel data set as it contained the largest number of samples. Normalization of the raw data sets was performed using MAS5 algorithm followed by quantile normalization. TO The mesenchymal proliferation and neurogenesis modules are known to define prognostic subtypes of glioma. In contrast, the proastrocytic module represents a novel signature, as only a few individual expression markers of glioma proastrocytic differentiation have been reported so far The other subtype was characterized by short survival. Notably, in all the datasets, patients with proastrocytic tumors survived on average several fold longer than those with tumors of the mesenchymal, proliferative, and other subtypes. Thus, the proastrocytic subtype is strongly and reproducibly associated with favorable prognosis. Conclusion: In summary, we explored the structure