ccd 短波近红外光谱快速检测牛奶中掺假物质的研究 - 食品科学.pdf

※分析检测 食品科学 2009, Vol. 30, No. 06 135 1 2 1 1 1, 王右军 ， 朱大洲 ，屠振华 ，庆兆珅 ，籍保平 * (1. 中国农业大学食品科学与营养工程学院，北京 100083 ；2. 中国农业大学信息与电气工程学院，北京 100083) 采集了25 个合格液态牛奶样品，并制取了3 组分别掺入不同含量掺假物质的牛奶样品：水解植物蛋白粉 (0.3～50g/kg)，乳清粉(0.03～30g/kg)，植脂末(0.03～30g/kg)，每组含50 个样品。应用CCD 短波近红外光谱仪(600～ 1100nm)扫描其透反射光谱，研究利用近红外光谱快速定量检测牛奶中掺假物质的可行性。采用偏最小二乘法建立 近红外光谱与牛奶中掺假物质含量之间的定量模型。结果表明，对掺入的水解植物蛋白粉的定量预测准确度较高， 相关系数为0.969 ，预测标准差为0.456g/kg ，可以满足定量检测的需要。预测乳清粉和植脂末含量时，由于很多 样品的掺假物浓度低于1‰时，受近红外光谱检测限的限制，难以准确预测牛奶中这两种掺假物的含量。 近红外；牛奶；水解植物蛋白粉；乳清粉；植脂末 Study on Rapid Determination of Adulterated Components in Milk by Charger-coupled Device Shortwave Near-infrared Spectrometer 1 2 1 1 1, WANG You-jun ，ZHU Da-zhou ，TU Zhen-hua ，QING Zhao-shen ，JI Bao-ping * (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China； 2. College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China) Abstract ：In this study, 25 normal milk samples without any adulterated components were collected, and then 3 groups (50 samples/group) of adulterated milk samples were prepared by addition of different concentrations of hydrolyzed vegetable proteins (HVP, 0.3 to 50 g/kg), whey powder (0.03 to 30 g/kg), and non-dairy creamer (0.03 to 30 g/kg), respectively. A charger- coupled device (CCD) near-infrared (NIR) spectrometer (600 to 1100 nm) was applied to scan the permeable reflection spectrum, the possibility of rapid detection of adulterated components in milk was investigated, and the qu