超高静压在琥珀酸生产菌株选育中的应用.pdf

微生物学通报 APR 20, 2008, 35(4): 561~564 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 简 报 超高静压在琥珀酸生产菌株选育中的应用 姜 岷* 陈可泉 蔡 婷 吴 昊 韦 萍 ( 210009) 摘 要: 采用超高静压对一株产琥珀酸放线杆菌 A3 进行诱变育种, 进一步提高其生产性能。考察 了压力、变压速度、生长期对菌株致死率的影响。在压力为200 MPa, 50 MPa/min 变压速度的诱 变条件下对稳定期菌株进行诱变, 筛选到一株突变菌株产琥珀酸放线杆菌 B19, 琥珀酸产量达到 32.2 g/L, 比出发菌株 A3 提高了 17.9%, 代谢副产物乙酸的产量降低了 11.5%, 经过 6 次传代表明 突变菌株具有稳定的遗传特性。 关键词: 超高静压, 产琥珀酸放线杆菌 B19, 琥珀酸, 诱变育种 The Application of Ultra High Hydrostatic Pressure in the Screening of Succinic Acid Producing Strain * JIANG Min CHEN Ke-Quan CAI Ting WU Hao WEI Ping (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009) Abstract: Actinobacillus succinogenes A3 was mutagenized by ultra high hydrostatic pressure for improv- ing the production ability of succinic acid. The effects of pressure, rate of pressure changes and growth pe- riods on the lethality rate of the strain were investigated. The strain in stationary phase was mutagenized at 200 MPa with the pressure changes at the rate of 50 MPa/min. As a result, a mutation strain Actinobacillus succinogenes B19 was obtained, and the concentration of succinic acid could reach 32.2 g/L, which was 17.9% higher than that of the original strain, and in the meanwhile the concentration of acetic acid wa