基于紫外光谱的花生油掺伪检测 - 食品科学.pdf

186 2012, Vol. 33, No. 06 食品科学 ※分析检测 杨 晨，于修烛 * ，王 昕，桂海龙 (西北农林科技大学食品科学与工程学院，陕西 杨凌 712100) 为建立一种花生油掺伪检测方法，利用花生油和其他食用油在紫外光谱特征吸收峰不同，建立定量检测 模型及并对模型进行验证分析。结果表明：花生油与其他食用油在 280nm 处特性吸收存在差异性，利用这一特性 建立花生油掺伪定量分析模型，其方程为y ＝46.72x －27.51(y 为掺伪比例/% ；x 为吸光度) ，相关系数为0.9779 ， 标准差为 3.099% 。盲样验证预测值与实际掺伪值高度线性相关，相关系数为0.9841 ，标准差为3.849% ，检出限 约为 10%，相对误差均小于 15% 。紫外光谱花生油掺伪快速检测方法是可行的。 花生油；掺伪；紫外光谱；检测 Ultraviolet Absorption Spectroscopy for the Detection of Adulteration in Peanut Oil YANG Chen ，YU Xiu-zhu* ，WANG Xin ，GUI Hai-long (College of Food Science and Engineering, Northwest A F University, Yangling 712100, China) Abstract ：An ultraviolet absorption spectroscopic method for detecting adulteration in peanut oil was developed. Based on differences in the ultraviolet characteristic adsorption peaks between peanut oil and other edible oils, a quantitative detection model was established as follows: y ＝46.72x －27.51 [y ＝adulteration ratio (%, m/m); x ＝absorbance]. The correlation coefficient of the model was 0.9779 and the standard deviation 3.099%. A highly linear correlation was observed between the predicted and actual adulteration ratios of an unknown sample with a correlation coefficient of 0.9841 and a standard deviation of 3.849%. The detection limit was approximately 10% and the relative error was less than 15%. The ultraviolet absorption spectroscopic method is applicable for the rapid detection of adulteration in peanut oil. Key words ：peanut oil ；adulteration ；ultraviolet absorption spectroscopic method ；detection 中图分类号：TS227 文献标识码：A 文章编号：1002-6630(2012)06-0186-04 花生油含油酸、亚油酸以及白藜芦醇等活性成分， 大了推广的难度[ 1 1 ] 。紫外光谱法具有快速、准确的特 营养价值丰富且风味独特，深受广大消费者喜爱[1] 。花