兔颈动脉球囊成形术后白细胞介素6、c反应蛋白变化的实验研究.doc

兔颈动脉球囊成形术对白细胞介素6和超敏C反应蛋的影响 王正龙 石 蓓* (遵义医学院 第一附属医院 心内科，贵州 遵义563003) 摘要：目的　评价血清超敏C-反应蛋白(hs-CRP)与白细胞介素-6(IL-6)与血管损伤程度及再狭窄的关系。方法 36只新西兰兔，通过球囊损伤右颈动脉建立血管损伤模型，根据球囊损伤程度分为：假手术组、轻度球囊损伤组和重度球囊损伤组。于术后即刻取血管标本作伊文斯蓝染色，初步判断血管损伤程度，术后1月行病理检查判断血管增生程度；每组均于术前、术后1天、7天和28天抽血作hs-CRP与IL-6测定；IL-6采用ELISA法测定，hs-CRP采用免疫浊度法测定。结果 ①伊文斯蓝染色见：假手术组未被染色，轻度球囊损伤组内皮被染成不均匀兰色，重度球囊损伤组被染成较均匀兰色；病理检查见：假手术组未见内膜增生，轻度球囊损伤组见内膜轻度的增生，重度球囊损伤组见内膜明显的增生。②术前3组血清hs-CRP和IL-6水平无明显差别；hs-CRP与IL-6水平均显著性升高（P 0.05）；P 0.05）。术后28天IL-6水平在球囊损伤组均显著高于假手术组，重度球囊损伤组明显高于轻度组球囊损伤组（P 0.05）；术后28天时血清hs-CRP水平基本恢复正常，三组间比较无明显差别。结论 血清hs-CRP与IL-6可能与血管损伤程度有关，可作为判断血管损伤程度的指标之一，而IL-6还有可能直接参与了血管损伤后的远期血管重塑过程。 关键词 超敏C-反应蛋白；6；颈动脉；Effect of Injury of Blood Vessel on Serum Levels of hs-CRP and IL-6 After Rabbit Carotid Artery Balloon AngioplastyShi Bei Wang Zhenglong (Dept. Cardiology, The First Affiliated Hospital of Guizhou Zunyi Medical University, Zunyi 530021, China) Abstract：Objective To explore the relationship high-sensitivity C-reactive [基金项目]贵州省优秀科技教育人才省长专项资金项目，黔省专合字（2006）47号 [通讯作者]石蓓*(1964-),女，教授，研究方向：心脏病介入E-mail:shibei2147@ [作者简介]王正龙(1980-)，男，硕士研究生. E-mail:wangzhenglong111@ protein (hs-CRP) and interleukin-6 (IL-6) and degree of vascular injury and late restenosis after vascular injury. Methods the right carotid artery was injured by balloon to establish vascular injury model, according to the degree of balloon injury carotid artery,36 New Zealand rabbits were randomly divided into 3 groups: mild balloon injury group,severe balloon injury group and sham group. Evans blue staining of blood vessels Intima was immediately done after vascular injury, preliminary to determine the extent of blood vessels damage, and pathological examination was made at 28 day after blood vessels damage to determine the extent of angiogenesis; Serum levels of hs-CRP and IL-6 were detected at pre-operation,and at 1 day, 7 day and 28 day after blood vessels injury by immuno-nehelomitery and ELISA method,Respectively. results ① Evans blue staining: Intima of blood ve