(法医学课件）脑库建设与神经病理学.pptx

脑库建设与神经病理学;;1. Brain tissue - different brain areas - paraffin embedded immunostainings - frozen Q-PCR, microarrays, proteomics - fresh cell cultures 2. Cerebrospinal fluid - ventricular CSF biomarker reserach, proteomics 3. DNA - from blood genetics – coupled to pathology 4. Extensive neuropathological report 可以提供临床病理报告 Clinical data are available Information about time of death, post-mortem delay, brain weight, pH CSF 5. Special requests possible 研究人员可以提出特殊要求 ;CNS disorders represented in tissue samples collected in BrainNet Europe centres;BrainNet Europe;供者填表;对无自主能力者，由家属填表;Autopsy protocals 取脑程序;;;;;;样本取材与诊断的标准化;神经病理诊断;Misfolded proteins disturbed degradation and aggregation;Primary Neurodegenerative disorders Molecular diagnosis 神经系统变性疾病的分子诊断;AD- Braak stages for amyloid are quite rough: Stage A : senile plaques in parahippocampal and frontobasal regions Stage B: in between stage A and C Stage C: neuritic plaques everywhere, also in the occipital cortex ;“老年性痴呆” ？ AD- Braak stages for neurofibrillary tangles (nft) Stage 1: few nft in parahippocampal gyrus PHG Stage 2: in between stage 1 and 3 Stage 3: many nft in PHG and cornu Ammonis field 1 (CA1) Stage 4: stage 3 plus many nft in temporal neocortex Stage 5: stage 4 plus many nft in frontal and/or parietal neocortex Stage 6: stage 5 plus many nft in occipital neocortex ; Braak stages for Lewy body pathology in Parkinson’s disease诊断的准确性 ;Multiple sclerosis (MS) 诊断的进一步细化;样本储存 (冰冻标本和石蜡切片）;临床资料与档案管理;数据库与样本提供;研究者研究项目申请表 （正规化）;;样本的使用;欧洲脑库的侧重点;;;;Familial AD Global atrophy, mostly frontal, temporal and parietal and hippocampus;脑标本的取得