复元醒脑汤对糖尿病脑梗死大鼠脑组织SDF-1尧 - 中国中医急症.PDF

国 医急症 2016 年8 月第25 卷第8 期 JETCM. Aug. 20 16 Vol . 25 No.8 1457 - - 研究报告 复元醒脑汤对糖尿病脑梗死大鼠脑组织SDF-1尧 CXCR4尧VEGF 基因及蛋白表达作用的研究鄢 吟 俊逸 方邦江 凌 丽 陈振翼 王晓翠 上海中医药大学附属龙华医院袁上海 200032 冤 图分类号院R285.5 文 标志码院A 文章编号院1004-745X 渊2016 冤08-1457-04 doi 院10.3969/j.issn.1004-745X.20 16.08.001 摘要铱 目的 观察复元醒脑汤对糖尿病脑梗死大鼠脑组织SDF-1尧CXCR4尧VEGF 基因及蛋白表达的干预作 用遥 方法 120 只健康雄性SD 大鼠随机分为正常对照组尧假手术组尧模型组尧治疗组各30 只遥 造模完成后 治 疗组予以复元醒脑汤 假手术组尧模型组予以0.9%氯化钠注射液 灌胃7 d 遥 测定比较各组实验大鼠脑组织 SDF-1尧CXCR4尧VEGF 的mRNA 及蛋白表达水平遥 结果 各组脑组织SDF-1尧CXCR4 尧VEGF 三种基因及蛋白 表达具有类似趋势 由高到低依次为正常对照组尧治疗组尧模型组及假手术组遥 正常对照组高于假手术组曰治 疗组高于模型组渊均P 0.01 冤遥结论 复元醒脑汤可上调SDF-1尧CXCR4 及VEGF 的mRNA 及蛋白表达水平 改善受损血管内皮功能及修复功能而促进局部血管再生和侧支循环建立遥 关键词铱 糖尿病脑梗死 复元醒脑汤 SDF-1 CXCR4 VEGF Experimental Study of Fuyuan Xingnao Decoction on the Gene Expression and Protein Secretion of SDF- 1 CXCR4 and VEGF in Diabetic Cerebral Infarction Rats SHEN Junyi FA NG Bangj iang LING Li et al. Longhua Hosp ital Shanghai Unive rs ity of Traditional Chinese Medic ine Shanghai 200032 China 揖Abstract 铱 Obj ective 院 To carry out the effect of Fuyuan Xingnao Decoction on the gene expression and protein secretion of SDF-1 尧CXCR4 and VEGF in diabetic cerebral infarction rats. Methods 院 Randomly all the enrolled healthy male SD rats were divided into 4 groups as Group C 渊control 冤 Group AD 渊treated with placebo after acute cerebral infarction combined with diabetes mellitus 冤 Group DM 渊diabetes mellitus without acute cerebral infarc鄄 tion 冤 and Group AT 渊treated with Fuyuan Xingnao Decoction after acute cerebral infarction combined with dia鄄 betes mellitus 冤. After 7 day the gene expression and protein secretion of SDF-1 尧CXCR4 and VEGF were mea鄄 su