响应面法优化春雷霉素发酵培养基及培养条件.pdf

584 中国抗生素杂志2014年8月第39卷第8期 响应面法优化春雷霉素发酵培养基及培养条件 张泽生1祖玉姣1，2，+余悦3雷萌萌3董硕，田欢3杨帅，孙东s (天津科技大学食品工程与生物技术学院，天津300457) 养条件相关因素的显著性进行评价，以春雷霉素效价为响应值，筛选出3个可信度90％以上的因素：黄豆粕粉量、装液量和接种 量为重要影响因素。经最陡爬坡实验确定中心点范围后，利用CCD设计及响应面分析，最终确定培养基的最优组合为：黄豆粕 提高到T3670Hg／mL，相比优化前效价提高了56．17％。 关键词：春雷霉素；响应面；培养基：培养条件 中图分类号：R978．1+2文献标志码：A offermentationmedium for Optimization andconditions SUrlace proouctionByresDonsemetnoaologv andSun ZhangZe—shen91，ZuYu-jia01’。，YuYue3，LeiMeng—men93，DongShu03，Tian Shuai3 Huan3，Yang Don93 ofFood and ofScienceand (DepartmentEngineeringBiotechnology,TianjinUniversity Technology,Tia巧in300457) Abstract surface usedto fermentationmediumandconditionsfor Responsemethod(RSM)wasoptimize wasusedtoevaluatetheinfluenceofrelatedfactorsandchoosed kasugamycin．Firstly,Plackett—Burman(PB)design titerof as value．Itshowedthatthreefactors the rolesinthemediumand kasugamycinresponse playingimportant conditionswere of in of selected，f．e．addition medium．thevolum andinoculationamountof SOybeanpower liquid wasmorethan ascentwas to Streptomyceskasugaensis．whosecredibility 90％．Secondly,thesteepestpath employed determinethe themedium of andconditionsforthefuturecentral CCD optimalregion and surface wereusedtodeterminethebestcombination．whichiSadditionof in response analysis