hplc 凝胶过滤色谱法测定牛奶及其乳制品中的a - 河北师范大学学报.pdf

36 / 1 / 河北师范大学学报/ / Vol. 36 No. 1 2012 1 JOURNALOFHEBEI NORMAL UNIVERSITY/ Natural Science Edition/ Ja n. 2012 HPLC 凝胶过滤色谱法测定牛奶及其 * 乳制品中的 A- 乳白蛋白和 B- 乳球蛋白 1 2 1 1 1 3 王 浩 , 蒋利群, 石振华 , 常彦忠, 段相林 , 张志国 ( 1. , 050024; 2. , 450008; 3. ( ) , 011500) : , A- B- . 1. 0, , 90 % , 0 . 4 % ~ 3. 1 % . , , , , , . : HPLC ; ; ; ; A- ; B- : O 658 : A : 1000- 5854( 2012) 01- 0091- 05 Isolation and Quantitative Assay of A-lactalbumin and B-lactoglobulin from Milk and Dairy Products by Gel Colume High Performance Li uid Chromatography 1 2 1 1 1 3 WANG Hao , J ANG Liqun , SH Zhenhua , CHANG Yanzhong , DUAN Xianglin , ZHANG Zhiguo ( 1. College of Life S cience, Hebei Normal U niversity , H ebei S hijiazhuang 050024, China; 2. School of Xinxiang Sanit ation, Henan Xinxiang 450008, China; 3. China M engniu Dairy Company Limited, Neimenggu Huhehaote 011500, China) Abstract:T o est ablish a method based on gel colume hig h performance liquid chromat ography ( HP LC) . Wit h this met hod, A-lact albumin and B-lact oglobulin of milk and dairy product s can be quant it at ively analyzed. T he chromat og raphic resolution of each component is near 1. 0 and baseline separat ion is achieved . T he recovery rate is more t han 90 % and t he value of relative standard deviat ion ( RSD) is 0. 4 % ~ 3. 1 % . T he sam ple