牛分支杆菌与肺结核分支杆菌基因组的比较 - 现代生物医学进展.pdf

Progress in Modern Biomedicine 2006 Vol. 6 No. 7 # 67 # X 牛分支杆菌与肺结核分支杆菌基因组的比较 徐广贤 赵德明 ( 北京 100094) : 通过比较基因组学的方法研究发现, 牛分支杆菌与肺结 杆菌基因组的同源性为99. 95% , 但在牛分枝杆菌基因组中 有11 个缺失区, 大小从1kb 到 12. 7kb, 遗传信息的缺失引起牛分枝杆菌的基因组减小; 牛分枝杆菌与肺结 分枝杆菌H37Rv 间 存在着2437 个单 苷酸多态性( SNPs) , 与肺结 分枝杆菌CDC1551 间存在着2423 个单 苷酸多态性( SNPs) , 牛分支杆菌与肺结 分枝杆菌在编码细胞壁和分泌蛋白上变异程度也是巨大的研究结果揭示了牛分支杆菌与肺结 分枝杆菌的遗传关系, 为研 究分支杆菌疫苗和诊断试剂提供理论依据, 对牛肺结 病的防治有着非常重要的意义 : 牛分支杆菌; 肺结 分支杆菌; 比较基因组学; 单 苷酸多态性( SNPs) : S855. 1 : A Comparision of genome between Mycobacterium bovis and Mycobacterium tuberculosis X U Guang - xian, ZHAO De- ming (National Animal TSE Laboratory, College of eterinary Medicine, ChinaAgricultural University, Beij ing 100094, China) ABSTRACT Th ough the compa ision of genome between the Mycobacte ium bovis and Mycobacte ium tube culosis, it is concluded that the homology of Mycobacte ium bovis and Mycobacte ium tube culosis a e 99 . 95% espectively, but the e a e 11 deletants( ange, f om 1kb to 12. 7kb in the genome of the Mycobacte ium bovis. The absence of genetic info mation esulted in eduction of genome in Mycobacte ium bovis. The e a e 2437 single- nucleotide polymo phisms ( SNPs) between the Mycobacte ium bovis and Mycobacte ium tube culosis H37Rv, 2423 SNPs, be- tween the Mycobacte ium bovis and Mycobacte ium tube culosisCDC1551. The extent of va iation is obvious on Codocyte cellwall and sec eted p o- tein between the two. The finding eveals the genetic elationship between Mycobacte ium bovis and Mycobacte ium tube culosis, which offe s a theo etical basis fo