魔芋葡甘露寡糖的干燥加热硒酸化及其产物的抗氧化性.PDFVIP

※基础研究 食品科学 2013, Vol.34, No.05 5 魔芋葡甘露寡糖的干燥加热硒酸化及其 产物的抗氧化性 1,2 1 1 1 1,* 和智坤 ，赵改红 ，李梦婷 ，王晓燕 ，李灿鹏 (1.云南大学化学科学与工程学院，云南 昆明 650091；2.丽江师范高等专科学校生命科学系，云南 丽江 674 100) 摘  要：在亚硒酸存在的条件下，采用干燥加热的方法对魔芋葡甘露寡糖(KOS)进行硒酸化；并用红外光谱、紫 外光谱、X射线衍射等技术对硒化魔芋葡甘露寡糖(Se-KOS)进行表征，同时测定Se-KOS 的抗氧化性。红外光谱实 - 1 验结果表明：Se-KOS在890cm 附近出现Se=O 的吸收峰，证明硒已被成功接到KOS 中。与KOS相比，Se-KOS 的 + 2+ ABTS ·清除能力、DPPH 自由基清除能力、还原能力和Fe 螯合能力等体外抗氧化能力均有显著增强，证明硒酸化 对提高KOS的抗氧化性来说是一种很有效的方法。 关键词：魔芋葡甘露寡糖；干燥加热硒酸化；表征；抗氧化性 Selenization of Konj ac Glucomannan Oligosaccharides by Dry Heating in the Presence of Selenite and Antioxidant Activity of Selenized Products 1,2 1 1 1 1,* HE Zhi-kun ，ZHAO Gai-hong ，LI Meng-ting ，WANG Xiao-yan ，LI Can-peng (1. School of Chemical Science and Technology, Yunnan University, Kunming 650091, China ； 2. College of Life Science, Lij iang Teachers College, Lij iang 674 100, China) Abstract ：In the present study, konj ac-glucomannan oligosaccharides (KOS) were selenized by dry-heating in the presence of selenite, and selenized KOS (Se-KOS) were characterized by ultraviolet, infrared (IR) and X-ray diffraction spectroscopy. Furthermore, the in vitro antioxidant capacity of Se-KOS was evaluated. The IR spectrum of Se-KOS showed a strong band -1 at 890 cm attributed to Se=O, suggesting that selenium has been successfully conjugated with KOS. The DPPH radical