加味大黄蟅虫丸治疗慢性粒细胞白血病慢性期的临床及实验研究.pdfVIP

提 要 本研究目的在于通过临床观察和体外实验两部分的研究，探讨分析加味大黄蟅虫 丸治疗慢性粒细胞白血病慢性期的临床疗效和作用机制，为中医药治疗慢粒提供一定 的临床疗效支持和理论依据。 本研究分为临床观察和体外实验两部分。临床观察主要是观察实验组(加味大黄 蟅虫丸加干扰素、羟基脲)与对照组(干扰素加羟基脲)治疗 CML 慢性期的临床疗效， 结果发现实验组在血液学缓解率、Ph 染色体阳性率、中医证侯积分、缩脾效果、骨 髓增生度等方面的疗效均优于对照组。表明加味大黄蟅虫丸加干扰素、羟基脲治疗 CML不仅能提高患者血液学缓解率，而且对细胞遗传学缓解亦有一定的作用，还可以 明显改善慢粒慢性期患者的症状体征，提高生活质量。体外实验分别通过 MTT 法、 TUNEL 法检测加味大黄蟅虫丸含药血清对白血病 K562 细胞的增殖抑制、诱导凋亡作 用，结果表明加味大黄蟅虫丸含药血清不仅可抑制K562细胞增殖，而且可诱导K562 细胞凋亡。 加味大黄蟅虫丸经过初步的临床和实验研究证实可能是治疗CML的良药，能够提 高CML患者的临床治疗效果，值得进一步的深入研究。 关键词 慢性粒细胞白血病；加味大黄蟅虫丸；K562细胞；细胞凋亡 Clinical and Experimental Study on Chronic Myeloid Leukemia- Chronic Phase by Adding Flavored Sting Rhubarb Worm Pills Speciality：Oncology, Hematology; Integrated traditional chinese and western medicine in clinical Author ：Liu Lijuan Tutor：Sun Changgang Abstract Through clinical observation and vitro assay,the auter analyses and probes into the curative effect and mechanism of Adding Flavored Sting Rhubarb Worm Pills curing the Chronic Myeloid Leukemia-Chronic Phase,for traditional chinese medicine curing the Chronic Myeloid Leukemia provide the clinical curative effect and theoretical basis support. This research includes clinical observation and vitro assay two parts. Clinical observation approves that Adding Flavored Sting Rhubarb Worm Pills combined with interferon and chemotherapy has completely cure rate, and found that the experimental group in hematology remission rate, Ph chromosome positive, Chinese medicine syndrome integral, spleen and bone marrow shrinkage effect hyperplasia degree, etc are better than the curative effect of interferon and chemotherapy group.The vitro assay app