不稳定性心绞痛血瘀证的血浆蛋白质组学研究plasma proteomics .pdf

2009 年第67 卷 化 学 学 报 Vol. 67, 2009 第2 期, 167～173 ACTA CHIMICA SINICA No. 2, 167～173 ·研究论文· 不稳定性心绞痛血瘀证的血浆蛋白质组学研究 赵慧辉 王 伟* (北京中医药大学研究生院 北京100029) 摘要 为了寻找冠心病不稳定性心绞痛血瘀证血浆差异表达蛋白, 探索冠心病不稳定性心绞痛血瘀证的蛋白质组学特 点, 采用差异凝胶双向电泳和质谱联用技术对 12 例冠心病不稳定性心绞痛血瘀证患者和 12 例健康人血浆进行比较研 究. 初步发现了Fibrinogen β chain, Fibrinogen γ chain, α1-Antitrypsin, Haptoglobinβ chain, Haptoglobin α2 chain 在冠心病 不稳定性心绞痛血瘀证患者中高表达, ApoA-IV, ApoA-I, Transthyretin, ApoJ 在冠心病不稳定性心绞痛血瘀证患者中低 表达. 差异表达蛋白根据功能可分为以下三类: (1)急性时相反应负相蛋白; (2)载脂蛋白; (3)凝血相关蛋白. 冠心病不稳 定性心绞痛血瘀证可能与炎症反应、脂代谢紊乱以及凝血功能异常相关. 关键词 心绞痛; 血瘀证; 差异凝胶电泳; 蛋白质组; 生物标志物 Plasma Proteomics Study on Unstable Angina Patients with Blood Stasis Syndrome Zhao, Huihui Wang, Wei* (Graduate School of Beijing University of Traditional Chinese Medicine, Beijing 100029) Abstract To seek differentially expressed plasma proteins of unstable angina patients with blood stasis syndrome, two-dimensional difference gel electrophoresis and mass spectrometry were used to screen the differentially expressed plasma proteins among such patients with blood stasis syndrome and healthy volun- teers. Fibrinogen β chain, fibrinogen γ chain, α1-antitrypsin, haptoglobin β chain, haptoglobin α2 chain were significantly highly expressed in the plasma of these patients, while ApoA-IV, ApoA-I, transthyretin and ApoJ were decreased in their plasma. These identified increased expressed proteins could be divided into three categories according to their functions: (1) acute ph