fret 的理论基础及应用.pdfVIP

中国病理生理杂志 Chinese Journal of Pathoph siolog 2004, 20( 9) : 1721- 1724, 1738 # 1721 # [ ] 1000- 4718(2004)09- 1721- 05 # # FRET * , , # ( 第一军医大学病理生理学教研室 全军休克微循环重点实验室, 广东 广州 510515) The theory and application of fluor escence resonance ener gy tr ansfer SUN Xue- gang, ZHANG Li- hua, JIANG Yong# (Department of Pathophysiology and Key Laboratory for Shock and Microcirculation of PLA, First Military Medical Uni ersity, Guangzhou 510515, China) =A Review The structural and functional stud of protein is a major topic of current functional ge2 nomics. Fluorescence resonance energ transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in i o. FRET is an ideal technolog for detection of protein confor2 mation and protein- protein interaction b using fluorescence protein, traditional organic d es and other d es as probes. It uses fluorescence protein, traditional organic d es and other d es as its probes. The application ofFRET in the determination of intracellular events would be helpful for us to understand the structure and function of biolog molecules. [ ] 荧光共振能量转移; 蛋白质相互作用 [ KEY WORDS Fluorescence resonance energ transfer ; Protein interaction [ ] R363 [ ] A 2001年, 5 自然6 5科学6 杂志先后公布了人类 效地产生振荡偶极子, 与近处的受体探针的偶极子 基因组的工作草图, 人类从此进入了功能基因组时 产生共振。这种偶极子与偶极子之间的相互作用将 代。目前, 研究生物大分子, 特别是蛋白质的结构、 供体荧光团的能量非放射性地转移到受体荧光团。 功能、特点及其相互作用已经成为生命科学的工作 对于特定的供体- 受体对, 供体丢失的能量就是受 重点, 随着蛋白质研究的进展, 一项古老的技术- 荧 体得到的能量。FÊrster 显示这个过程的效率依赖于 光共振能量转移(fluorescence resonance energ transfer, 供体 受体间距离六次幂的倒数: E= 1/ {1+ ( R/ FRET) , 在生物学领域又增添了新的活力。 6