海地瓜多糖中蛋白含量测定方法比较 - 食品科学.pdfVIP

※分析检测 食品科学 2011, Vol. 32, No. 02 201 宿 玮，常耀光，薛长湖 * ，武晓琳 ( 中国海洋大学食品科学与工程学院，山东 青岛 266003) 比较4 种常用的蛋白定量方法来测定海参多糖中蛋白含量，建立海参多糖中蛋白含量的测定方法。氨基 酸组成折算出海地瓜多糖中蛋白含量为(5.06 ±0.31)% ，将此结果作为其他测定方法比较的标准。采用4 种常用的 蛋白定量方法：Folin- 酚法、BCA 法、考马斯亮蓝法、荧光光度法进行测定，对测定结果进行比较分析。Folin- 酚法、BCA 法、荧光光度法测定多糖中蛋白含量分别为(4.92 ±0.20)% 、(6.29 ±0.51)% 、(3.88 ±0.25)% 。考马斯 亮蓝在反应中不显色，此法检测不出多糖中蛋白。经比较分析，Folin- 酚法精确度高，重现性好，测定时间短， 操作简便，对仪器要求低，可以准确简便地测定海地瓜多糖中蛋白含量。 海参；海地瓜多糖；糖胺聚糖；蛋白定量；Folin- 酚法 Comparison of Four Methods for Determining Protein Content in Crude Acaudina molpadioides Polysaccharides SU Wei，CHANG Yao-guang ，XUE Chang-hu* ，WU Xiao-lin (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China) Abstract：Protein content in crude polysaccharide extract isolated from Acaudina molpadioides was determined comparatively by four commonly used protein quantification methods, i.e., Lowry, bicinchoninic acid, Bradford and fluorescence spectromet- ric methods in order to find out an accurate and simply method for quantifying protein in the matrix. It was calculated on the basis of amino acid composition that the crude extract contained (5.06 ± 0.31)% of protein, which served as a benchmark for the comparison among protein quantification methods. Lowry, bicinchoninic acid fluorescence spectrometric methods gave protein contents of (4.92 ± 0.20)%, (6.29 ± 0.51)% and (3.88 ± 0.25)%, respectively, while no protein was detected by Bradford method. The comparative results show that Lowry method is the most accurate method among the four quantification methods. Moreover, the method has the other characteristics of good reproducibility, short time consumption, simplicity and low requirements for analytical instruments and