小球藻糖蛋白的分离纯化与性质测定 - 食品科学.pdfVIP

※基础研究 食品科学 2006, Vol. 27, No. 11 101 小球藻糖蛋白的分离纯化与性质测定 1,2 1, 3 2 魏文志 ，夏文水 * ，李湘鸣 ，吴玉娟 (1.江南大学食品学院，江苏 无锡 214036； 2.扬州大学动物科学与技术学院，江苏 扬州 225009；3.扬州大学医学院，江苏 扬州 225009) 摘 要：小球藻经热水抽提、脱蛋白、乙醇沉淀得粗糖蛋白，粗糖蛋白经Sephadex-75 层析得到糖蛋白纯品。糖 蛋白纯品经Sephadex-200 层析证明为单一物质。紫外可见光谱只出现280nm 和195nm 的单峰。SDS- 聚丙烯酰胺凝 胶电泳显示糖蛋白分子量为63.7kDa。气相色谱分析表明该糖蛋白单糖组成主要是葡萄糖、半乳糖、鼠李糖。β- 消去反应证明糖和蛋白是O- 糖肽键连接的。 关键词：小球藻；糖蛋白；分离纯化；性质 Isolation,PurificationandCharacterizationoftheGlycoproteinfromChlorellaPyrenoidosa 1,2 1, 3 2 WEI Wen-zhi ，XIA Wen-shui *，LI Xiang-ming ，WU Yu-juan (1.School of Food Science and Technology,Southern Yangtze University, Wuxi 214036, China； 2.College of Animal Science and Technology,Yangzhou University, Yangzhou 225009, China； 3 Medical College, Yangzhou University, Yangzhou 225009, China) Abstract：The crudeglycoproteinwas extractedfromChlorellaPyrenoidosa with hotwater, andprecipitatedby ethanolafter deproteinization.ArefinedglycoproteinswerepreparedafterfurtherpurificationonSephadex-75columnchromatography,and theirhomogeneitywas examinedwith Sephadex-200 columnchromatography.Theultraviolet spectrashowedtheircharacteris- tic absorption peak at 280.00nm and 195.00nm. The molecular weight was estimated to be about 63.7kDa on SDS. It was proved that themain monosacchrideswere glucose, galactoseandxyloseby gas chromatography,andO-glycosidic linkagewas foundby β-eliminationreaction. Key words： Chlorella Pyrenoidosa；glycoprotein；isolation and purification；characterization 中图分类号：Q816 文献标识码：A 文章编号：1002-6630(2006