清营解表合剂对流感病毒APR8H1N1感染小鼠免疫机制的探究.pdfVIP

提 要 目的：探讨阴分伏热体质外感采用透伏解表法，即以解表祛邪为主，清透阴分伏 热以调体质为辅的治疗方法的作用机理。 方法及指标：①理论探讨部分主要从中西医对流行性感冒的病因病机及治疗的不 同点为切入点，进行中西医对比，总结中医药治疗流行性感冒的独特优势。以此为基 础，重点探讨阴分伏热体质外感形成原因、作用机理、治疗思想、方药分析及其临床 意义。②实验研究主要采用半体内法、MTT法、流式细胞术等技术及实验方法，以清 营解表合剂干预流感病毒 A/PR/8/H N 感染小鼠，通过观察其死亡保护率、肺组织病 1 1 理形态学改变、肺指数、肺组织病毒血凝滴度以及检测免疫器官胸腺指数和脾指数的 大小，验证其整体抗病毒的疗效；通过观察对造模小鼠淋巴细胞增殖功能和对小鼠全 血中Th1/Th2调节的影响，探讨其发挥作用的免疫机制。 结果：①清营解表合剂对病毒感染小鼠有死亡保护作用，能显著改善感染小鼠的 肺组织病变程度，降低肺指数、血凝滴度。②清营解表合剂能通过提高淋巴细胞增殖 功能和调节全血中Th1/Th2平衡来提高流感病毒感染小鼠的免疫功能，从而对病毒感 染小鼠起保护作用。 关键词 流感；清营解表合剂；A/PR/8/H N 病毒；全血；Th1/Th2； 1 1 Research on the Function of Qingyingjiebiao Mixture on the Immunological Mechanism of Rats which was Infected by Influenza Virus A/PR/8/H1N1 Speciality: clinical basis of TCM Author: Jiang Lu Tutor: Jiang Jian-guo Abstract Object: In the early stage of common cold, the method of clearing the ying fen and relieving the exterior which mean taking the relieving exterior as the main and cleaing the ying fen to regulate the constitution as the assistant was disscussed to be used to the patient with the constitution of having latent heat in yin fen. Method and index: ① Theoretical research: the modern and ancient documents were carded. the difference of treating common cold and the view of its cause and pathogenesis between Western Medicine and Traditional Chinese Medicine was taken as the starting point. The advantages on treating common cold with traditional Chinese medicine was summarized. The thoughts of therapy on people with latent heat in yin fen constitution who take common cold was disscussed. The herbs in Qingying jiebiao mixture were analyzed. ② Experimental research: the effect of th