二株假单胞菌的疏水性及其对菲的降解能力 - 环境科学学报.pdfVIP

第 24 卷第 5 期 环 　境 　科 　学 　学 　报 Vol . 24 ,No . 5 2004 年 9 月 ACTA SCIENTIAE CIRCUMSTANTIAE Sep . ,2004 ( ) 文章编号 2004 　　　中图分类号 :X131 　　　文献标识码 :A 二株假单胞菌的疏水性及其对菲的降解能力 (南京农业大学生命科学学院 农业部农业环境微生物工程重点开放实验室 , 盛下放 , 何琳燕 , 龚建勋　 南京 　210095) ( 摘要 :从石油污染土壤中分离纯化到2 株能以菲为唯一碳源和能源的细菌 GF2 和 GF3 ,初步鉴定均为假单胞菌属 Pseudo monas) 细菌. 根据细菌在烃 - 水两相体系中的细胞数量研究了 GF2 和 GF3 菌株的表面疏水性及其在不同环境条件下的变 化 , 同时对 GF2 和 GF3 在液体培养条件下降解菲的效能进行了研究. 结果表明, GF2 和 GF3 表面疏水性随培养时间、温度及 ( ) ( ) ( - 1) p H 值的变化而变化 , GF3 的疏水性 748 % 大于 GF2 586 % . 在 25 ℃下培养 7d , GF2 和 GF3 对菲 250mg ·L 的降解率分 别达到 856 %和 100 %. 关键词 :假单胞菌 ; 菲 ;疏水性 ; 降解 The hydrophobicity of t wo strains of pseudomonas and their degradation of phenanthrene ( SHENG Xiafang , HE Linyan , GONGJianxun 　College of Life Science , Nanjing Agricultural University ; Key Lab of Microbio logical Engineering of Agricultural Environment , Ministry of Agriculture Nanjing 　210095) Abstract :Two bacterial strains that could utilize phenanthrene as sole carbon and energy sources were isolated from oilcontaminated soils. The two strains were identified as Pseudomonas based on their morphological and physiobiochemical characteristics. The surface hydrophobicity of the two strains and its affecting factors were studied based on the cell amount of bacteria in hydrocarbonwater twophase system. The degrada tion of phenanthrene by GF2 and GF3 were carried out in solution . The results showed that the hydrophobicity of the two stra