内皮祖细胞的分离培养与鉴定 - 生物技术通报 - 中国农业科学院.pdf

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 2 1, 2 3 2 1 1 白春雨 侯玲玲 庞全海 关伟军 马月辉 1 2 ( , 100194; , 030801; 3, 100044) : 内皮祖细胞的分离方法有免疫磁珠分离法淋巴细胞分离 分离法( 1. 077)和差速贴壁法, 这 3种方法已被人 们广泛使用, 均可分离到一定的目的细胞分离到的目的细胞在培养过程中逐渐分化成熟发育为内皮细胞在内皮细胞 + + + 和内皮祖细胞的鉴别区分,使用 CD34 /CD133 /KDR 鉴定为内皮祖细胞, 同时使用内皮祖细胞吞噬 DilacLDLFITCUEA双 阳性的方法也可鉴定为内皮祖细胞 : 内皮祖细胞 分离 鉴定 Isolation, Culture and Identification of Endothelial P rogenitor Cells 1, 2 3 2 1 1 ai Chunyu Hou L ingling Pang Quanhai GuanW eijun M aYuehui 1 ( Institute of Anmi alScience, ChineseA cademy of AgriculturalScience, B eij ing 100193; 2 College of A nimalScience and VeterinaryM edicine, Shanx iAgriculture University, aigu 030801; 3 Institute of Lfie Science and B ioengineering, B eij ing Jiaotong University, B eij ing 100044) Abstract: There are three isolation methods of endothelial progenitor cells, including mi munomagnetic beads, lymphocyte separa ting medium( 1. 077) and differential adhesion, which has been w idely used and could isolate a certain number of the desired cells. + These cells gradually differentiate, mature, and develope into endothelial cells in culture process. In identification, cellsw ith CD34 / + + CD133 /KDR are identified a