抗凝剂（Anticoagulant）.docVIP

抗凝剂（Anticoagulant） Anticoagulant control There are many kinds of anticoagulants and different properties. They must be chosen according to the inspection so as to achieve the desired results. The commonly used anticoagulant and its usage are briefly described as follows: 1, EDTA (EDTA) salt EDTA has two sodium, two potassium and three potassium salt. Can be used with calcium node become chelate, thereby preventing blood coagulation. EDTA salt is very small on the red and white cells, according to the international standards committee of Hematology (International committee standard of public hematology, ICSH 1993) recommends two EDTA potassium blood cell count as anticoagulant, the dosage of EDTA-K2-2H2O1.5-2.2MG (4.45 + 0.85 mol) /ml blood. The effects of EDTA-NA and EDTA-K2 on blood cell count were small, but the solubility of two sodium was obviously lower than two potassium, sometimes affecting anticoagulant effect, and other anticoagulants were not suitable for blood cell count. Table 1-1 is the effect of several anticoagulants on white cell count, EDTA effect of platelet aggregation, not suitable for blood coagulation tests and platelet function tests. 2. sodium oxalate (sodium, oxalate) oxalate and calcium ions in the blood calcium oxalate precipitation, thereby preventing blood coagulation. 3. heparin (heparin) heparin is widely located in almost all tissues and vessels, such as lung, liver, spleen and so on. It is a kind of mucopolysaccharide containing sulfuric acid group. It is a dispersed substance with an average molecular weight of 15000 (2000-40000). Heparin can enhance antithrombin III (AT- III) inactivation of serine proteases, thereby preventing thrombin from forming, against thrombin and preventing platelet aggregation. Anticoagulation per milliliter requires 15 + 2.5IU. Although heparin can keep red blood cells due to its natural form, but often can cause leucocyte aggregation with smear on Roche () produces a blue background staining, so anticoag