两种不同的上游法处理精液对常规体外受精结果的影响（The effect of two different upstream methods on semen on conventional ivf results）.doc

两种不同的上游法处理精液对常规体外受精结果的影响（The effect of two different upstream methods on semen on conventional ivf results） Conventional in vitro fertilization papers: two different upstream methods for the effect of semen on conventional ivf results Date: December 28, 2010 [abstract] objective: to explore the effect of two different upstream methods on the results of conventional in-vitro fertilization (IVF). Methods: a total of 287 cycles, this study will be patient couple, the wife eggs about such as divided into two, the male semen is divided into two copies of the same amount of upstream method optimization and separately using two sperm, and the separation of sperm and egg line of conventional in vitro fertilization (ivf). The fertilization of the fertilized eggs was observed and the fertilization rate, cleavage rate and high quality embryo rate were compared. Results: the total fertilization failure of the centrifugation group was 72.4%, the cleavage rate was 91.2%, and the high quality embryo rate was 52.5%. The total fertilization failure of the upstream group was 12 cycles, the accuracy rate was 75.1%, the cleavage rate was 93.2%, the high quality embryo rate was 53.9%, and the differences in the control indexes were not found to be statistically significant (P 0.05). Conclusion: the two upstream methods have no statistically significant effect on the results of fertilization, but the upstream method should be superior to the centrifugal method. [keywords] conventional in vitro fertilization; The upstream method; Fertilization failure The results of fertilization with two different sperm swimming - up methods ZHANG Hong1, MA Bo2 (1. Department of Postgraduate, Guangxi Medical University, Nanning 530021, China; 2. Reproductive Center of Police Hospital, Shenzhen 518029, China) [Abstract] Objective: To compare the results of in-vitro fertilization with two different swim-up methods to separate sperm and co-culture with oocytes. Methods: Retrospective analysis 287 cycles re