荷叶中槲皮素提取工艺研究.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2008，36(14)：5705-5706 责任编辑 理雪莲 责任校对 马君叶 荷叶中槲皮素提取工艺研究 雍国新 (海口经济学院应用技术学院，海南海口570203) 摘要 的]优化荷叶提取槲皮素的工艺。【方法]研究了3种提取方法对槲皮素转移率的影响；通过对荷叶中槲皮素的回流提取条 件进行4因素3水平正交试验，确定了荷叶中槲皮素的最佳提取工艺。【结果]采用乙醇回流提取法提取荷叶中槲皮素效果最佳，槲皮 素转移卒均高于60％，明显优于乙醇温浸法(16．2％)和碱温浸法(20．0％)。4因素对于槲皮素转移率的影响程度依次为固液比溶 剂浓度提取时间提取温度。荷叶中槲皮素最佳提取工艺条件为：乙醇回流提取，乙醇溶液浓度60％，提取温度100℃，料液比1：20， 提取时间1 ho『结论1经试验验证，该研究筛选优化的工艺条件基本稳定、可行。在此工艺条件下，槲皮素的转移率在70％xX_E。 关键词 荷叶；黄酮；槲皮素；提取工艺 中图分类号 $682．33 文献标识码 A 文章编号 0517—6611(2008 J14-05705-02 Study on Extraction Technique of Quercetin from Lotus Leaf YONG Guo-xin fCollege of Applied Technology，Haikou Economic Polytechnic College，Haikou，Hainan 570203) ． Abstract『Objective1 This study aimed to optimize extraction technique of quercetin from Lotus le ．[Method]The effects of 3 kinds of extraction methods on transfer rate of quercetin were mvestigated．Through the I』9(34)orthogonal design experiment about conditions of reflux extraction，the oDtimum extraction process of quercetin from lotos leaf was ascertained．【Result]The ethanol refluxing method of extracting quercetin from L．1e could get optimum effect，by which the transfer rate of quercetm‘was more than 60％，being obviously higher than that by ethanol warm immersion (16l2％)and alkali warm．immersion f20．0％1，resp．The influencing degree of 4 factors on transfer rate in order was．ratio of solid to liquid solvent densitvextr8etion timeex~acfion temperature．The optimum process conditions for extracting quercetin from L．1e were the ethanol refluxing txtraction，60％ethano1．extraction temperature of 100℃，solid-liquid ratio of 1：20，extraction time of 1 h． 【Conclusion]The extraction technic『ue screened bv this study was stable and feasible on the whole，under which，the transfer rate of quercetin was over 70％． Key words ．Lotus leaf：Flavonoids：Quercetin：Extraction technique 睡莲科植物莲( Nelumbo l~itcifela Gaertn．)的干燥叶具有 1．3．2 槲皮素含量的测定。 清热解暑，升发清阳，凉血止血的功效嗍。