动物细胞培养常用技术（Common techniques for animal cell culture）.doc

动物细胞培养常用技术（Common techniques for animal cell culture） Common techniques for animal cell culture Animal cell culture Laboratory Animal Center of Zhongshan University Preface In 1885, Roux first tried to culture tissue in vitro. The material was chick embryo neural plates, using saline as the culture medium, and the term tissue culture was first used. In 1907, Harrison and 1912 Carrel began using tissue culture as a method for studying the culture of isolated animal cells. Because of the application of tissue culture in medical research, such as the production of antiviral vaccines, it has gradually developed into a sophisticated technology. Foster many cell lines, cell line success, especially in the tumor cell lines established for various materials, such as Hela cell lines (Gey, 1952), can be used to carry out a series of research, more to promote the development of tissue culture technology. Hot research areas in tissue culture 1) intracellular activities such as DNA replication and transcription, protein synthesis, and energy metabolism; 2) flow within the cell, such as RNA from the nucleus to the cytoplasm, translocation of the hormone receptor complex; 3) ecology, such as nutrition, infection, virus or chemical mutagenesis, drug action; 4) the interaction between cells and cells, such as embryonic induction and kinetics of cell populations. Classification and basic concepts of tissue culture Sort: 1. tissue culture (Tissue Culture) refers to the removal of tissue from the body, in the simulated physiological environment, sterile, appropriate temperature and nutrition conditions, to survive and grow and maintain its structure and function. 2. cell cultures (Cell, Culture) cultures are individual cells and cell populations. 3. organ culture (Organ, Culture) is a method of culturing, growing, and maintaining certain functions of organs, primordia, organs, or organs throughout the body. Cell biological characteristics of tissue culture: The difference between the 1