饲料型四倍体刺槐幼胚离体培养挽救技术研究.pdfVIP

第23卷 第2期 河 北 林 果 研 究 V01．23 No．2 2 0 0 8年6月 lI口 Icl J0I】RNAI 0F 碾 『I’RY AND 0】 HAIlD砌 姗 JUn．2008 文章编号：1007-4961(2008}02．0113．05 饲料型四倍体刺槐幼胚离体培养挽救技术研究 贺佳玉，李 云，姜金仲，郝 晨，曹春伟 (北京林业大学生物科学与技术学院林木花卉遗传育种教育部重点实验室，北京 100083) 摘要：遗传变异丰富、具有重要遗传育种价值的饲料型四倍体刺槐幼胚自然败育率很高，研究其离体培养挽救 技术具有重要的遗传育种学意义。以处于不同发育时期的饲料型四倍体刺槐幼胚为材料，进行了其离体培养 促萌试验，试验结果表明：胚珠的适宜离体培养时期为花后30 d，萌动率47．7％；胚珠的适宜萌动培养基为 Nitsch+6一BA 0．5 ms／L+IBA 2 mg／L+G 0．5 mg／L；筛选出的最佳愈伤组织诱导培养基上得到的愈伤组织诱 导率为38．6％，并初步诱导出了不定芽；愈伤组织切片表明，饲料型四倍体刺槐幼胚愈伤组织的增殖生长以 内起源为主。 关键词：饲料型四倍体刺槐；幼胚促萌；胚败育；组织培养 中图分类号：S 791．27 文献标识码：A Saving young embryo of tetraploid Robinia pseudoacacia for fodder by embryo culture／n HE Jh·yu，LI Yun，JIANG Jin·Zhong，HAO Chen，CAO Chun-wei (Key Laboratoryfor Genetics and Breeding in Forest Trees and Ornamental Plants， Ministry ofEducation，＆溉 Forestry University， 100083，China) Abstract：The abortion rate of young embryo，which originates from varied genetic material and plays an important role in b1ack locust breeding，oftetraploid Robiniapseudoacacia is high．It is necessary to work out how to improve germination of ． these ahortional young embryos by culture in vitro for black locust breeding．Therefore the test to save abortional young em— bryo~which were in different development stages，oftetraploid Robinia pseudoacacia by culture／n v／tro was conducted．The results indicated that(1)The suitable stage for ovule culture was 30 days after being pollinated and the embryos’culture— response—rate was47．7％；(2)The suitable development substrates for ovule cultare／n v／tro was Nitsch+6一BA 0．5ms／ L+IBA 2 ms／L