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Chapter11DNA Replication and Synthesis Semiconservative replication of DNA makes genetics continuity between parental and progeny cells possible, as predicted by Watson-Crick model.Each strand of the parent helix serves as a template for its complement. DNA synthesis is a complex but orderly process orchestrated by a myriad of enzyme and other molecules. Together, they function with great fidelity to polymerize nucleotides into polynucleotide chains. Other enzymes interact with DNA, leading to genetic recombination. Key topics in this chapter 11.1 The mode of DNA replication 11.2 synthesis of DNA in microorganisms 11.3 DNA synthesis : A model 11.4 A coherent mode of DNA synthesis 11.5 Genetic control of replication 11.6 Eukaryotic DNA synthesis 11.7 DNA replication ,telomeres and telomerase 11.8 DNA recombination The Meselson-Stahl Experiment In 1958, Matthew Meselson and Franklin Stahl published the results of an experiment providing strong evidence that cells use semiconservative replication to produce new DNA molecules. Escherichia coli cells were grown for many generations in a medium where I5NH4C1 (ammonium chloride) was the only nitrogen source. A heavy isotope of nitrogen, I5N contains one more neutron than the naturally occurring 14N isotope. Unlike radioactive isotopes, 15N is stable and thus does not decay (i.e., it is not radioactive). Semiconservative Replication in Eukaryotes In 1957, the year before the work of Meselson and his colleagues was published, J. Herbert Taylor, Philip Woods, and Walter Hughes presented evidence that semiconservative replication also occurs in eukaryotic organisms. They experimented with root tips of the broad bean Vicia faba, which are an excellent source of dividing cells. These researchers examined the chromosomes of these cells following replication of DNA. They monitored the replication process by labeling DNA with 3H-thymidine, a radioactive precursor of DNA, and then performing autoradiography. Autoradiography is