血必净对脓毒症大鼠心肌保护作用探究.docVIP

血必净对脓毒症大鼠心肌保护作用探究【摘要】 目的观察血必净注射液对脓毒症大鼠心肌损伤的保护作用。方法 将30只SD大鼠随机分为假手术组(Sham组)、脓毒症模型组(CLP组)及血必净治疗组(XBJ组)，每组10只。Sham组只开腹关腹，余同CLP组；CLP组采用盲肠结扎穿孔法造模；XBJ组造模后立即经腹腔注射血必净注射液8 ml/kg。观察24 h后检测血清肌钙蛋白I(TnI)、脑钠肽（BNP）、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β，留取左心室心肌组织观察超微结构的改变。结果 ①脓毒症状态下TnI、BNP、TNF-a、IL-1β水平显著升高，应用血必净注射液后各项指标明显改善。②血必净组心肌组织超微结构的损害较脓毒症组明显减轻。结论 血必净注射液能改善脓毒症大鼠的心肌损伤，其机制可能与下调TNF-a、IL-1β水平有关。? 【关键词】脓毒症；血必净注射液；心肌损伤；炎症因子；组织结构?? Protective effect of Xuebijing injection on myocardiac injury in rats with sepsis QUE Gan-bo，DENG Xing，XIAO Xiong-jian,et al．Intensive Care Unit, The Second Hospital of Longyan, Fujian 364000,China ? 【Abstract】 ObjectiveTo investigate the protective effect of Xuebijing injection on myocardiac injury in rats with sepsis，and the possible mechanism of such effect．Methods Thirty SD rats were selected and randomly divided into sham operation group(Sham)，sepsis group(CLP)and treatment group with Xuebijing injection(XBJ)，with 10 rats in each group．In sham group，only open-close abdomen was performed．In CLP group，cecal ligation and puncture(CLP)was performed to induce sepsis．Xuebijing injection(8 ml/kg)was administered via celiac in XBJ group after CLP．Serum concentrations of troponin-I(TnI)，brain natriuretic peptide(BNP) ，tumor necrosis factor-α(TNF-α) and interleukin(IL)-1β were measured．Ultrastructure changes in myocardium were examined．ResultsThe levels of TnI，BNP，TNF-α and IL-1β were increased significantly in rats with sepsis，and were decreased by Xuebijing injection in rats with sepsis．There were less myocardial ultrastructure injuries in Xuebijing group than in sepsis group．Conclusion Xuebijing injection improves myocardiac injury in rats with sepsis，possibly through down-regulating serum TNF-α and IL-1β levels．? 【Key words】Sepsis; Xuebijing; Myocardiac injury; Inflammatory factor; Histomorphology ? 脓毒症是严重创伤、感染、休克、大手术后常见的并发症。具有发病率、病死率、住院费用高的特点。心肌细胞损伤是脓毒症的常见并发症之一，并可致其他器官功能障碍。血必净注射液是由天津急救医学研究所研制的防治脓毒症多器官功能障碍综合征(multiple organ dysfunction syndr