二补助育改良方对小鼠子宫内膜胞饮突LIFER和-环球中医药杂志.PDF

环球中医药2017年4 月第10卷第4期摇 Global Traditional Chinese Medicine,April2017,Vol郾10,No郾4 摇摇 425 ·论著 · 二补助育改良方对小鼠子宫内膜胞饮突、 LIF、ER和PR表达的影响 石玥摇 穆国华摇 吴丽婷摇 申萌萌摇 赵青竹摇 刘雁峰 揖摘要铱摇 目的摇 观察二补助育改良方对胚泡着床障碍模型小鼠子宫内膜胞饮突、白血病抑制 因子(leukemia inhibitory factor,LIF)、雌激素受体(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)表达的影响。方法摇 建立小鼠胚泡着床障碍模型,将50 只实验动物分为5组:空白组、 模型组、西药组1(补佳乐组)、西药组2(阿司匹林组)、二补助育改良方组,每组10 只。 造模与给药 同时进行,其后处死小鼠,扫描电镜观察胞饮突的表达,并采用免疫组化HBP法检测小鼠子宫内膜 LIF、ER、PR的表达量。结果摇 扫描电镜下,二补助育改良方组小鼠子宫内膜表面形态较规整,大量 微绒毛及丰富胞饮突表达,细胞之间的间隙明显。 免疫组化结果示,二补助育改良方组小鼠子宫内 膜LIF、ER、PR表达较模型组明显增加(P0.05);LIF表达低于补佳乐组(P0.05),ER表达优于补 佳乐组(P0.05),PR表达与补佳乐组无明显差异(P0.05);各指标与阿司匹林组比较无明显差异 (P0.05)。结论摇 二补助育改良方可能通过调节胚泡着床期子宫内膜胞饮突、LIF、ER和PR 的表 达,从而提高子宫内膜容受性,参与胚泡植入的黏附过程,改善着床障碍,创造良好的受孕环境,促 进胚泡着床。 揖关键词铱摇 二补助育改良方;摇 子宫内膜容受性;摇 胞饮突;摇 白血病抑制因子;摇 雌激素受 体;摇 孕激素受体 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2017郾04郾010 Effect ofErbu Zhuyu improving prescription on pinopodes,LIF,ER and PR expression in mice endometrium摇 SHI Yue,MU Guohua,WU Liting,et al. 摇 Department of Ggynaecology, Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Corresponding author:LIU Yanfeng,Email::liuyaf8888@ 揖Abstract铱摇 Objective摇 To observe the effect of Erbu Zhuyu improving prescription on pinopodes, LIF,ER and PR expression in endometrium of mice model with dysfunctional embryo implantation. Methods摇 50 experimentalanimalswereusedtoestablishmodelofdysfunctionalembryoimplantation,and the micewere divided into five groups (10 in each group):blank group,model group,western medicine treated group 1 (progynova group), western medicine treated group 2 (aspirin group), Erbu Zhuyu improving prescription group. Pinopodes in uterine endometrium were examined by scanning electron microscopy. LIF,ER and PR expressions were detected by immunohistochemistry. Results摇 Scanning electron microscopy