玉米简单重复序列不对等交换的热点区域定位.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(5): 958−961 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00958 玉米简单重复序列不对等交换的热点区域定位 汤继华1,2 马西青1 滕文涛 1 严建兵 1 戴景瑞1 李建生1,* 1 , 100193; 2 , 450002 : , 22 (RIL), SSR , 40 SSR , 0.34%~14.63%, 10−2~10−1, (AG)n 58.3%31 11 , 9 , 3 5 2 : ; (SSR); ; Mapping Unequal Crossing over Hotspot Region of Simple Sequence Repeat in Maize 1,2 1 1 1 1 1,* TANG Ji-Hua , MA Xi-Qing , TENG Wen-Tao , YAN Jian-Bing , DAI Jing-Rui , and LI Jian-Sheng 1 National Maize Improvement Center of China, China Agricultural University, Beijing 100193, China; 2 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China Abstract: The polymorphism of simple sequence repeat (SSR) in biological genome is one result of unequal crossing over for homologous chromosomes; therefore it has theoretical importance in clarifying the hotspot regions of unequal crossing over. A set of recombinant inbred lines (RILs) population that derived form an elite hybrid Yuyu 22 was used in this study, its genetic com- ponents of the population was analyzed by means of SSR analysis, and 40 unequal crossing over SSR markers were found. The −2 −1 frequency of the unequal crossing over in the RIL population was 0.34–14.63%, with 10 –10 frequency per generation, and the (AG)n repeat SSR markers accounted for 58.3%. There were 31 unequal crossing over markers locating on 11 chromosomal hot- spot regions,