基于遥测技术对巴马小型猪部分生理指标的观测 - 中国实验动物学报.doc

基于遥测技术对巴马小型猪部分生理指标的观测 陈亮1,2，潘永明2，朱科燕2，陈方明2，徐孝平2，陈民利2 (1.浙江大学动物科学学院,杭州 310000；2.浙江中医药大学动物实验研究中心，比较医学研究中心，杭州 310053) [摘要] 目的 应用遥测技术观察巴马小型猪在清醒自由状态下心电、血压、呼吸、活动等指标昼夜波动变化。方法 取雄性6月龄巴马小型猪只，行浅表股动脉VAP血管通路植入手术，恢复d后，用EMAK遥测系统进行24h连续清醒自由心电、血压、呼吸、活动指标监测，并用EMAK分析软件对上述指标进行分析。结果6月龄巴马小型猪白昼心率显著高于黑夜心率（P0.01）P0.05，P0.01），白昼平均心率为76.22次/分，黑夜平均心率为67.03次/分，白昼平均PR间期、QRS间期和QT间期分别为109.97ms、42.72ms、380.37ms，黑夜平均PR间期、QRS间期和QT间期为112.32ms、44.01ms、389.24ms。巴马小型猪白昼收缩压、舒张压、平均压都显著高于夜间（P0.01）129.57mmHg、96.75 mmHg、111.73mmHg，夜间平均收缩压、舒张压、平均压分别为122.81 mmHg、92.65 mmHg、106.19mmHg，且黑夜收缩压、舒张压、平均压下降率分别为19.89%、19.05%、19.35%。另外，巴马小型猪在白昼的活动情况与呼吸频率都要显著高于夜间（P0.01）。结论遥测技术可以对清醒自由状态下巴马小型猪心电、血压、呼吸、活动等进行连续监测，能真实的反应小型猪在24h内上述生理指标的变化规律，。 [关键词] 遥测技术；巴马小型猪；心电；血压 [中图分类号] [文献标识码] A [文章编号] hysiological parameters in Bama minipigs by telemetry technology CHEN Liang1,2, PAN Yong-ming2, ZHU Ke-yan2,CHEN Fang-ming2,XU Xiao-ping2,CHEN Min-li2 (1,Zhejiang University College of Animal Science,Hangzhou 310000,China 2.Zhejiang Chinese Medical University Laboratory Animal Research Center/ Comparative Medical Research Center, Hangzhou 310053, China) [Abstract] Objective Measure the circadian rhythm of ECG, blood pressure, respiration, activities in Bama minipigs by telemetry technology. Methods Six 6-month-old Bama minipigs were implanted with VAP port in Superficial femoral artery, after recovery 7 days , 24h consecutive monitoring electrocardiogram(ECG), blood pressure, activity and respiratory parameters were recorded and analysed in the EMAK noninvasive telemetry system. Results The ECG, blood pressure, respiration and activities in Bama minipigs showed circadian rhythm changes, Bama minipigs daytime heart rate was significantly higher than the nighttime heart rate (P0.01), and daytime PR interval , QRS interval and QT interval was significantly lower than the nighttime (P0.05, P0.01), daytime mean heart rate was 76.22 beats/min, nighttime mean heart rate was 67.03 night beats/min; daytime mean PR interval, QRS interval