真菌细胞壁做为愈伤敷材及化妆品之应用.ppt

組織再生Tissue Regeneration 生長因子 基因重組--PDGF 血小板---PDGF、VEGF、TGF-b1、 FGF 、EGF 生物醫學材料 無毒性、安全性 生物相容性、生物降解性 足夠之機械支撐力 促進組織再生 殺(抑)菌性 促進(保護)生長因子釋放(破壞) 生物醫學材料 膠原蛋白 玻尿酸 幾丁質、幾丁聚糖 PGL PGEL 利用真菌做為生物醫學材料Application of fungal materials Why fungal materials? Fungi provided wide range of products from food to drugs Cell wall composition Polysaccharides and chitin/chitosan (幾丁質、幾丁聚糖) 靈芝子實體細胞壁-SACCHACHITIN之應用 Fruiting bodies of Ganoderma ? Chemical Analysis化學分析 TLC GC GC/MS NMR SACCHACHITIN Is SACCHACHITIN safe and functional?安全性及有效性 Safety Cytotoxicity test, Immunogenecity Animals Rat Guinea pig Canine Inhibition Enzyme and Microbes Human/Clinic trials Cells 利用細胞測試毒性 Fibroblast (天竺鼠纖維母細胞) Keratinocyte (人類角質細胞) Corneal epidermis cell (角膜表皮細胞) 結果均顯示無毒性，並有促進增值之作用 人體試驗Patients Totally 47 patients were treated with SACCHACHITIN. All the patients suffered chronic skin ulcer for over 6 months with 3-10 cm wound in diameter. All the patients were treated with chemotherapy or other possible treatment, previously. 36 patients were with satisfactory healing. The other 11 patients were lost their contact. What is the mechanism that SACCHACHITIN functioned as a wound healing accelerator?One of the properties of fungal cell wall is to binding lytic enzymes in environment as a defense. Inflammation Early stage platelets aggregate blood coagulation Late stage Neutrophils, monocytes Macrophages---bacteria Matrix metallo-proteases (MMPs) MMPs were produced by Neutrophils. MMPs hydrolyzed collagen piling as well as the growth factors. 傷口面積變化 一種日本CHITINT商品 SACCHACHITIN 在Guinea pigs實驗也有類似結果 SACCHACHITIN 10%混合CMC軟膏 For pets 為寵物製造之 燙傷之流類犬 已使用在許多寵物 之案例 Day 1 Day 7 Day 15 Day 30 Day 13 Day 30 三月齡混種雌犬4kg體重，疑似因不當保溫電燈泡散發出的輻射熱所引致之三度燙傷。 一歲齡混種雌犬，疑似因天冷長時間睡在電熱器前造成在右髖部及大腿焦痂皮膚潰瘍的病變，大小約為9 cm、9 cm、7 cm的三邊長的三角形。 Day 14 Day 35 66 years old male Infection after open chest operation for 7 months Treatment of SACCHACHITIN 2001