塞来昔布对大鼠蛛网膜下腔出血后迟发性脑血管痉挛防治.doc

塞来昔布对大鼠蛛网膜下腔出血后迟发性脑血管痉挛防治[摘要] 目的 探讨塞来昔布(Celecoxib)对迟发性脑血管痉挛的防治。方法 36只SD大鼠随机分为对照组,蛛网膜下腔出血组,塞来昔布治疗组,每组12只。应用枕大池二次注血法建立大鼠SAH模型,治疗组第1次注血30min后给予塞来昔布灌胃,利用HE染色和透射电镜分别观察各组处理后第7天基底动脉形态学和超微结构改变。结果 (1)经塞来昔布治疗后,治疗组管径显著大于蛛网膜下腔出血组,管壁厚度显著小于蛛网膜下腔出血组(P0.05)。(2)塞来昔布治疗后痉挛血管的形态学和超微结构得到改善。结论 塞来昔布对蛛网膜下腔出血后迟发性脑血管痉挛有防治作用。 [关键词] 蛛网膜下腔出血; 迟发性脑血管痉挛; 塞来昔布; 炎症 [中图分类号] R364.1+3;R743.35 [文献标识码] A [文章编号] 1673-9701(2010)05-27-03 An Experimental Study on Prevention of Cerebral Vasospasm with Celecoxib in A Rats of Delayed Subarachnoid Hemorrhage GUANG Junrong TONG Haibo TANG Huoquan FAN Yimin LIU Yaoting HAO Jiehe Shanxi Medical University,Taiyuan 030001,China [Abstract] ObjectiveTo investigate the effect of Celecoxib on cerebral vasospasm of subarachnoid hemorrhage. MethodsThirty six Sprague-Dawley rats are randomly separated into control group,subarachnoid hemorrhage group(SAH) and SAH+Celecoxib group.There were twelve animals in each group. SAH were developed with two times injections of 0.3mL arterial blood into cisterna magna,and 0.3mL saline was injectted in group NS by using same method. Rats in therapy group were lavaged with Celecoxib in thirty minutes after. blood injection first. We use the radial wall thickenss and cross-sectional lumen area after SAH seven days as parameters under light,transmission electron microscopy examination. Results(1)In the SAH+Celecoxib group ,the artery lumens dilated and the vessel wall thinned markedly than that of SAH group,but no significance compare with control group.(2)Celecoxib treatment reduced ultrastructural and morphometric vasospastic changes. ConclusionThe study results prove that Celecoxib can prevent the cerebral vasospasm in rats model of subarachnoid hemorrhage. [Key words]Subarachnoid hemorrhage; Delayed cerebral vasospasm; Celecoxib; Inflammation 脑血管痉挛(cerebral vasospasm,CVS)是蛛网膜下腔出血(subarachnoid haemorrhage,SAH)患者致死和致残的主要原因之一,发病率达45%,如何有效防治是当今神经外科研究的热点。本实验以大鼠SAH为模型,应用形态学、透射电镜等方法探讨塞来昔布对SAH后迟发性CVS的防治,为临床应用塞来昔布治疗迟发性CV