家兔玻璃体液铁-磷含量变化及PMI相关性探究.doc

家兔玻璃体液铁\磷含量变化及PMI相关性探究[摘要] 目的 研究死后家兔玻璃体液铁、磷浓度变化与死亡时间的关系,寻找一种精确推断PMI的方法。方法 应用全自动生化分析仪检测样本中铁、磷含量,对所得数据进行统计学处理。结果 家兔死后54h内眼玻璃体铁、磷元素与PMI显著相关,其单元回归方程分别为y=14.48-0.244x(x=PMI,y=Fe,r=-0.993)、y=-4.573+0.366x(x=PMI,y=Fe,r=0.965)、y=0.137+0.041x(x=PMI,y=P,r=0.975)。结论 在54h内玻璃体液铁、磷含量变化可作为推断PMI的参考指标。 [关键词] 法医病理学; 死后经过时间; 玻璃体液; 离子浓度; 家兔; 全自动生化分析仪 [中图分类号] R919.1 [文献标识码] A [文章编号] 1673-9701(2010)03-07-02 Correlation between Postmortem Interval and Levels of Iron and Phosphorus in Rabbit Vitreous Humor:An Experimental Study LI Hai GAO Cairong College of Forensic Medicine of Shanxi Medical University,Taiyuan 030001,China [Abstract] ObjectiveTo investigate the correlation between postmortem interval(PMI)and the concentration of iron(Fe)and phosphorus(P)in vitreous humor in rabbits after death in an effort to seek a exact method of estimating PMI. MethodsThe levels of iron(Fe)and phosphorus(P)in the vitreous humor were measured by automatic biochemistry analyzer. ResultsThe iron(Fe)and phosphorus(P)levels in rabbit vitreous humor within 54h after death were related to PMI significantly. The formulate of the relationship between PMI and iron(Fe)level was y=14.48-0.244x(x=PMI,y=Fe,r=-0.993)and y=-4.573+0.366x(x=PMI,y=Fe,r=0.965)and the formulate of the relationship between PMI and phosphorus(P)level was y=0.137+0.041x(x=PMI,y=P,r=0.975). ConclusionThe levels of iron(Fe)and phosphorus(P)in rabbit vitreous humor within 54h may serve as a reference index for the estimation of PMI. [Key words]Forensic pathology; Postmortem interval; Vitreous humor; Concertration of ionic; Rabbit; Automatic biochemistry analyzer 死亡时间推定(esti mati on of the time sincedeath)是法医学尸体检验首先要解决的问题。本实验旨在探索一种简单、快速、稳定,益于普及和推广的方法,以德国Cobas Integra 400全自动生化分析仪为工具,利用比色法研究家兔玻璃体液内铁和磷含量变化,以找出其与死后时间(post mortem interval,PMI)的相关性,为进一步阐明死后经过时间的生物学效应提供实验性依据。 1 材料与方法 1.1 实验动物分组及取材 健康家兔150只(雌雄不限,重约1～2.5kg),随机分为10组,每组15只,在室内将兔断头处死后,于死后即时、6、12、18、24、 30、36、42、48、54h各取玻璃体液1mL,以3000r/min离心(4℃)2min,取0.5mL上清待测。