电针不同经穴对急性心肌缺血家兔血浆乙酰胆碱及去甲肾上腺素影响.doc

电针不同经穴对急性心肌缺血家兔血浆乙酰胆碱及去甲肾上腺素影响[摘要] 目的：比较电针不同经穴对急性心肌缺血家兔模型外周血血浆中自主神经递质去甲肾上腺素与乙酰胆碱的影响有无差异性，探讨不同经穴间作用的相对特异性。方法：青紫蓝家兔90只,随机选择10只作为正常对照组，余80只以股静脉静滴垂体后叶素造成急性心肌缺血模型,将复制成功的模型家兔随机分为模型组、电针“神门”组、电针“内关”组、电针“支正”组、电针“太渊”组、电针“三阴交”组,每组10只。ELISA法检测电针后血浆中去甲肾上腺素与乙酰胆碱的含量。结果：造模后外周血中去甲肾上腺素释放减少（P0.05）。结论：电针“内关”、“神门”对提高外周血中交感神经递质去甲肾上腺素释放、抑制乙酰胆碱释放的效果最为显著，电针“支正”的效果次之，而电针“太渊”、“三阴交”对上述指标影响较小。 [关键词] 急性心肌缺血；电针；去甲肾上腺素；乙酰胆碱 [中图分类号] R245.3 [文献标识码]A [文章编号]1673-7210（2008）08（b）-023-03 Effects of electroacupuncture at different acupoints on plasma acetylcholine and noradrenalin in rabbits with acute myocardial ischemia HE Lu1, LONG Di-he1, HU Ling2, TANG Xiao-min3, WU Zi-jian2, CAI Rong-lin2, WANG Ke-ming2, LIU Jing1 (1.2006 graduated student, Anhui College of Traditional Chinese Medicine, Hefei230038, China; 2.School of Acupuncture and Osteology, Anhui College of Traditional Chinese Medicine, Hefei230038, China; 3.Nanjing Gulou Hospital, Nanjing210008, China) [Abstract] Objective: To compare the different effects of electroacupuncture(EA) at different acupoints on plasma acetycholine and noradrenalin in rabbit models with acute myocardial ischemia(AMI), and investigate the relative specificity of different acupoints. Methods: A total of 90 healthy rabbits were selected, 10 were selected randomly and set as normal group. The other 80 rabbits were injected with pituitrin by femoral veins to establish acute myocardial ischemia models, and then the successful rabbit models were randomly divided into 6 groups: model group, EA at “Shenmen” group, EA at “Neiguan” group, EA at “Zhizheng” group, EA at “Taiyuan” group and EA at “Sanyinjiao” group, and each group had 10 rabbits. ELISA assay was employed to detect the contents of norepinephrine(NA) and acetylcholine(ACH). Results: The NA content in peripheral blood of rabbits decreased obviously after establishing AMI models(P0.05). Conclusion: Electroacupuncture at “Neiguan” and “Shenmen” has the most significant effects on enhancing the releas