乳酸羟基乙酸共聚物微泡声学造影剂的制备与评价试验研究李奇林.DOC

乳酸羟基乙酸共聚物微泡声学造影剂的制备与评估实验研究 李奇林 全学模 （重庆400014,重庆医科大学附属儿童医院,） [提 要] 目的 制备乳酸羟基乙酸共聚物(poly lactic-co-glycolic acid ,PLGA)微泡声学造影剂，考察其理化及声学特性。方法 复乳法制备不同PLA/PGA比例的微泡，考察其理化特性；体内外显影评价不同剂量、不同机械指数条件下微泡造影剂的声学信号强度变化规律。结果 PLGA微泡成品呈白色粉末状，以生理盐水或PBS稀释溶解后为乳白色混悬液；光镜观察显示微泡大小均匀，分散程度好； Coulter计数显示0.01g多聚体微泡冻干粉溶于10ml蒸馏水中的浓度为2×l09～Mechanical index, MI）几乎呈直线关系。兔心脏造影检查可观察到PLGA微泡造影剂能顺利通过肺循环实现左心显影。结论 PLGA微泡造影剂具有较高的可控性和稳定性，微泡共振频率较高，可以顺利通过肺循环实现左心室显影。 【关键词】 声学造影剂，乳酸羟基乙酸共聚物，理化性质 【中图法分类号】R445.1 Experimental Study on poly lactic-co-glycolic acid microbubble ultrasonic contrast agent preparation Li Qilin Quan Xuemo (Children’s Hospital of Chongqing Medical University,Chongqing 400014,China) [Abstract] Objectives To Prepare PLGA microbubbles and evaluate its physicochemical and acoustic properties.Methods We prepared PLGA microbubbles by double emulsion method .We examined its physicochemical properties and evaluated its acoustic properties in vitro under different doses with ultrasound energy at a variety of MI values. Result PLGA microbubbles were white powder and ivory suspension dissolved in saline or PBS. Under microscope, microbubbles had uniform sizes and good dispersion. The microbubble suspension contained 2.0×109～4.0×109 /ml microbubbles. The vitro study showed that acoustic intensity increased with PLGA microbubbles concerntration and changed with MI linearly. We observed that PLGA microbubbles had passed through pulmonary circulation and achieved left ventricular imaging in vivo. Conclusion PLGA microbubbles had an ideal controllability in preparation, outstanding stability and higher resonance frequency. The agents could successfully passed through pulmonary circulation and achieved left ventricular imaging.. [Key words] ultrasound contrast agent; poly lactic-co-glycolic acid; physicochemical properties [通信作者] 李奇林，E-mail: chenhj_1999@163.com 乳酸羟基乙酸共聚物（poly lactic-co-glycolic acid, PLGA）是获得FDA批准并应用于临床的生物医用材料，组织相容性好，可以在体内完全降解和代谢消失，无免