45S rDNA在小麦及其近缘物种染色体上的分布.pdf

HEREDITAS (Beijing) 2007 9 , 29(9): 1126―1130 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1126 45S rDNA 1, 2 1 1 1 1 徐川梅 , 别同德 , 王春梅 , 周波 , 陈佩度 1 210095 2 311300 : 将染色体C-分带和原位杂交技术相结合，系统研究了45S rDNA 在栽培一粒小麦、野生二粒小麦、普通 小麦、大麦、簇毛麦、硬簇麦、六倍体燕麦及鹅观草等物种染色体上的分布情况。这些物种染色体的次缢痕区 都有45S rDNA 位点, 某些非随体染色体上也有45S rDNA 位点分布。以小麦—鹅观草1Rk#1 二体附加系为材料， #1 通过顺序C 分带-FISH 技术首次将一个45S rDNA 定位到 1Rk 染色体短臂末端。 : 45S rDNA ；顺序C 分带-FISH 技术；小麦近缘物种 Distribution of 45S rDNA sequence on chromosomes of Triticum aes- tivum and its relative species 1,2 1 1 1 1 XU Chuan-Mei , BIE Tong-De , WANG Chun-Mei , ZHOU Bo , CHEN Pei-Du 1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Lab for Modern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300, China Abstract: Fluorescent in situ hybridization (FISH) and sequential C-banding and FISH techniques were used in locating 45S rDNA sequence on specific chromosomes of Triticum monococcum, T. dicoccoides, T. aestivum, Hordeum vulgare, Haynaldia villosa, T. durum-Haynaldia villosa amphiploid, Avena fatus, and Roegneria kamoji. High polymorphism be- tween common wheat and its relatives on 45S rDNA sequence distribution was detected. All the chromosomal secondary constriction regions of these species had 45S rDNA loci. Some non-satellite chromosomes had additional 45S rDNA loci. A 45S rDNA locus was located on the terminal region of the short arm of R. kamoji chromosome 1Rk#1 . Keywords: 45S rDNA; sequential C-banding and FISH; relative species rRNA5.8S rRNA28S rRNA5S rDNA , 60S40S ,