humanIL-2血清反向.docVIP

人工处理：以标准浓度取log值为横坐标，对应的logit值为纵坐标在普通坐标纸上或以标准浓度为横坐标，对应的B/B0为纵坐标在logit-log坐标纸上画出标准曲线（理想化时是一条直线）。根据待测样品的B/B0可以从坐标纸上查出样品的浓度值。如果使用普通坐标纸，查出的数值应取反对数才是最后的浓度值。 自动处理：使用logit-log或四参数数据处理模式，由电脑自动计算得出结果。 敏感度：g/mL； 图例 Human Interleukin 2 (IL-2) ELISA Kit 48 Tests Catalogue Number: E01I0308 Store all reagents at 2-8°C Valid Period: six months For samples: serum or blood plasma FOR LABORATORY RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! INTENDED USE This BG IL-2 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450nm using a spectrophotometer. In order to measure the concentration of IL-2 in the sample, this IL-2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-2 concentration. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. PRINCIPLE OF THE ASSAY The coated well immunoenzymatic assay for the quantitative measurement of IL-2 utilizes a polyclonal anti-IL-2 antibody and a IL-2-HRP conjugate. The assay sample and buffer are incubated together with IL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-2 concentration since IL-2 from samples and IL-2-HRP conjugate compete