甲基磺酸乙酯对烤烟种子发芽率的处理效应.PDFVIP

2011-06 ，32 （3 ） Chinese Tobacco Science 17 1,2 1 1 1,2 1 1 1* 1. 266101 2. 100081 EMSEMS 0.1%~1.3%EMS EMS 0.35%~0.52%1.3% EMS 87=97100K326 EMS S572.02 1007-5119201103-0017-04 DOI 10.3969/j.issn.1007-5119.2011.03.004 Mutagenic Effects of Ethyl Methane Sulfonate on Flue-cured Tobacco Seeds 1,2 1 1 1,2 1 1 WANG Junwei , JIANG Caihong , SONG Zhimei , ZHANG Li , WANG Lu , WANG Yuanying , 1* LIU Guanshan (1. Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of CAAS, Qingdao 266101, China; 2. The Graduate School of the Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract: In this study, tobacco seeds of different varieties were treated with the chemical mutagen, ethyl methane sulfonate (EMS). The germination rate and root lengths of the mutated seeds were measured. The results showed that germination was inhibited at EMS concentrations of 0.1%-1.3%, while the lower concentration EMS could increase the germination rate; the higher concentration EMS could significantly inhibit the germination and root elongation. By the selection criteria of lethal concentration 50, the appropriate concentrations of EMS for treating flue-cured tobacco seeds were 0.35%-0.52% and lethal concentrations for all five varieties were 1.3%. The sensitivity of tested tobacco varieties to EMS in descending order was Yunyan87=Yunyan97 Honghuada- jinyuan Zhongyan100 K326. Keywords: flue-cured tobacco; seeds; EMS; lethal concentration 50; mutant