麻醉喉镜污染状况和消毒方法探讨.docVIP

麻醉喉镜的污染状况及消毒方法探讨 摘要 目的 了解临床应用麻醉喉镜的污染状况，并结合临床探讨消毒方法的选择。方法 1. 用氨基比林试验法检测喉镜的残余血污染状况及从喉镜取样进行细菌培养了解其微生物污染状况；2.应用染菌方法比较超微软酸化水、2%戊二醛浸泡及高压蒸汽灭菌对污染喉镜的消毒效果。结果 1.喉镜柄和镜片的残余血污染率分别为48%及2.5%，微生物的综合污染率分别为96%及84%；2. 超微软酸化水及高压蒸汽均有很好的消毒效果。结论 临床应用麻醉喉镜污染状况严重，对其清洁消毒应予重视。用4%葡萄糖酸洗必泰清洗、干燥，然后以超微软酸化水浸泡10分钟对污染喉镜消毒效果确切。 关键词：麻醉喉镜；污染；消毒 Evaluation of the contamination and disinfection of anaesthetic Iaryngoscopes 【Abstract】 Objective To evaluate the contamination of the anaesthetic laryngoscopes and methods of disinfection of the laryngoscopes that are highly contaminated.Methods 1) Aminopyrine test was employed to determine the presence of occult blood and surveillance cultures to investigate the contamination on laryngoscopes.2)To compare the effect of disinfection of electrolysis soft oxidized water, 2% glutaraldehyde and autoclaving to the laryngoscopes was contaminated. Results. 1) The rate of handles and blades with occult blood is 48% and 2.5% respectively, the rate of contamination of handles and blades with microorganism is 96% and 84% respectively. 2) electrolysis soft oxidized water and autoclaving were found effective on disinfection of laryngoscopes . Conclusion The contamination of the anaesthetic laryngoscopes are in relatively high level . It must be given to pay attention for cleaning and disinfection. Immersion for ten minutes in electrolysis soft oxidized water after careful washing in 4% chlorhexidine gluconate is a valid way to disinfect anaesthetic laryngoscopes that are highly contaminated. 【Key Words】 anaesthetic Iaryngoscopes；contamination；disinfection 麻醉喉镜是直接与病人口咽部粘膜、唾液有时甚至是血液接触的侵入性操作器具，常规使用后，必将造成喉镜的污染，如清洁及消毒方法不当，则可能引起使用病人之间发生交叉感染。本文的目的在于了解我院经处理后准备提供给病人使用的麻醉喉镜的污染状况，并用染菌试验比较三种方法的去污消毒效果，以期找出一种既安全有效、又简便易行的消毒方法。 1. 材料与方法 1.1 材料 1.1.1 实验用麻醉喉镜 麻醉喉镜25套，其中麻醉科21套、急诊科2套、ICU2套，包括喉镜柄25个、喉镜片75个。所有污染状况调查采样均在应用者根据个人习惯对喉镜处理后提供给病人使用前进行。 1.1.2 实验菌 金黄色葡萄球菌(ATCC25923)、大肠杆菌（ATCC25922）、绿脓杆菌（ATCC27853）、Ⅱ型溶血性链球菌（临床分离）、白色念珠菌（ATCC14053）。 1.1.3 清洁消毒剂 4%葡萄糖酸洗必泰、2%碱性戊二醛（ci