高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量2.docVIP

高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量 张文静（湖北中医学院药学院02级本科药学（4）班，湖北 武汉 430064） 摘要：目的 建立HPLC同时测定双黄连粉针剂中绿原酸和黄芩苷外标定量测定方法。方法 用自制C8(C13混合型烷基键合硅胶柱(5(m，4.6mm(150mm)为固定相，甲醇和水溶液（用磷酸调pH2.7）为流动相，在C8(C13柱上梯度洗脱，流速为1mL?min-1，紫外检测波长为323nm。结果 绿原酸和黄芩苷的线性范围分别为4.0(100.0(g?mL-1和 8.6(215.0 (g?mL-1；相关系数分别为0.999 89和0.999 97；加样回收率分别为100.53%和99.94%；检测限分别为0.014 (g .mL-1和0.020(g .mL-1；精密度实验RSD分别为1.12%和 0.61%；重现性实验RSD分别为1.14%和0.73%，稳定性实验RSD分别为0.89% 和 0.54%；4批样品中绿原酸的含量在1.577(1.753 mg?mL-1，黄芩苷的含量在26.02(28.68 mg?mL-1。结论 该方法简便，快速，准确，灵敏度高，重现性好，成本低，可用于双黄连粉针剂的质量控制。 关键词：双黄连粉针剂；绿原酸；黄芩苷；高效液相色谱法，梯度洗脱 Quantitative Determination of Chlorogenic Acid and Baicalin in Shuanghuanglian Powder by HPLC ZHANG Wenjing ( Class 4 Grade 2002 of Undergraduate ,Dep. of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan 430064, China) ABSTRACT:OBJECTIVE To develop a RP-HPLC method for the determination of chlorogenic acid and baicalin in shuanghuanglian powder. METHODS A C8~C13mixedalkyl (5(m，4.6mm(150mm)bond silica for reversed-phase HPLC was prepared. The mobile phase was CH3OH―phosphoric acid solution (pH2.7). The flow rate was set at 1.0 mL/min. The detection wavelength was at 323nm. Chlorogenic acid and baicalin were separated by HPLC with grade elution. RESULTS:The linearity ranges of chlorogenic acid and baicalin were 4.0(100.0 (g /mL and 8.6(215.0 (g /mL respectively，the correlation coefficients were 0.999 89 and 0.999 97 ,the average recoveries of adding sample were 100.53% and 99.94%, detection limits were 0.014 (g /mL and 0.020 (g /mL. , the RSD (n=5) of measurement precision test were 1.12% and 0.61%, the RSD (n=5) of reproducibility between tests were 0.89% and 0.54%. The content of chlorogenic acid in Shuanghuanglian powder was 1.577(1.753 mg/mL，and that of baicalin was 26.02(28.68 mg/mL. CONCLUSION:The method was simple, fast, accurate，sensitive, reproducible and low cost. It is fit for the quality control of Shuanghuanglian powder. Key Words: shuanghuanglian powder ; chlorogenic acid ; baicalin；hig