果蝇卵巢滤泡细胞谱系发育中的功能性microRNA筛查.PDFVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2015, 37(5): 697–702 DOI: 10.11844/cjcb.2015.05.0356 研究简报 果蝇卵巢滤泡细胞谱系发育中的功能性microRNA筛查 * 赵 琨 李梦婕 辛天池 宣 涛 李明发 (上海交通大学生命科学技术学院, 上海 200240) 摘要 在果蝇发育过程中, microRNA(miRNA)作为负调控因子起着重要的作用。该文旨在 报 研究microRNA在果蝇卵巢滤泡细胞谱系中的功能, 该细胞谱系因易于体内遗传操作而成为研究 细胞命运决定与细胞迁移机制的良好模型。为了确认此过程中的功能性miRNA, 作者利用UAS/ GAL4二元表达系统对31个果蝇miRNA进行了表型筛选。结果表明, 若干miRNA可以在卵子发生 学 过程中引起多种严重表型。过表达与敲减miR-7均能阻断边界细胞迁移。miR-1 、miR-124和miR- 263b则在茎细胞诱导、边界细胞迁移或卵壳图式中发挥功能。结果表明, 该文所用基于UAS/GAL4 的方法可用于确认miRNA 的功能。 关键词 果蝇; 卵子滤泡细胞; microRNA; 细胞分化; 细胞迁移; 图式建成 学 A Screening for Functional microRNA in the Development of Drosophila Ovarian Follicle Cell Lineage 物 Zhao Kun, Li Mengjie*, Xin Tianchi, Xuan Tao, Li Mingfa (College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China) 生 Abstract As negative regulators of gene expression, microRNA (miRNA) plays important roles during Drosophila development. In this paper, we aimed to investigate the effects of miRNA on the commitment of Droso- phila ovarian follicle cell lineage, which is an in vivo genetically tractable model to understand the mechanisms of cell fate determination and cell migration. To identify functional miRNA in the process, we performed a phenotypic 胞 screening for total 31 Drosophila miRNA by UAS/GAL4 binary expression system. The results showed that several miRNA cause multiple severe defects during oogenesis. And both the overexpression and knock-down of miR-7 could block