弱精子症大鼠模型的建立及左旋肉碱对其改善作用的相关研究.docVIP

弱精子症大鼠模型的建立及左旋肉碱对其改善作用的相关研究 柳建明，姜辉，洪锴，白泉，赵连明，刘德风，马潞林 北京大学第三医院泌尿男科 摘要：目的：研究弱精子症大鼠模型的建立及左旋肉碱（L-肉碱）与精子质量的关系。方法：24只雄性SD大鼠随机均分成3组，分别连续灌胃20d，A组（对照组）：0.5% 羟甲基纤维素钠（溶剂）；B组：400mg/Kg奥硝唑悬液；C组：400mg/Kg奥硝唑悬液+100mg/Kg左旋肉碱，末次给药24h后，麻醉处死所有大鼠，分别检测各组精子密度、活力、形态正常率以及附睾总L-肉碱和游离L-肉碱浓度。结果：A组、B组及C组精子密度无明显差异（P0.05），A组、C组精子活力、精子形态正常率及附睾总L-肉碱、游离L-肉碱浓度明高于B组（P0.05），A组与C组精子活力、精子形态正常率及附睾总L-肉碱、游离L-肉碱浓度无统计学差异。精子活力与附睾总L-肉碱、游离L-肉碱浓度呈正相关（r=0.645,P0.05； r=0.676,P0.05），精子形态与附睾总L-肉碱、游离L-肉碱浓度呈正相关（r=0.557,P0.05； r=0.583,P0.05），相关性具有统计学意义。结论：奥硝唑可以降低大鼠精子活力、精子形态正常率，以及附睾L-肉碱水平，附睾L-肉碱浓度与精子活力、精子形态正常率呈正相关，L-肉碱对精子质量有改善作用。 关键词：弱精子症；左旋肉碱（L-肉碱）；奥硝唑；附睾 Establishment of Asthenospermia Rat Model and Research of the Role of L-carnitine Jian-Ming LIU, Hui JIANG, Kai HONG, Quan BAI, Lian-Ming ZHAO, De-Feng LIU, Lu-Lin MA Department of Urology and Andrology,Peking University Third Hospital,Beijing 100191,China Correspondence to: JIANG Hui,Email:jianghui55@163.com Abstract:Objective: To research the establishment of asthenospermia rat model and the relationship of L-carnitine between sperm quality.Metheds: Twenty-four male SD rats were randomly divided into three groups, 8in each. Group A (the control): 0.5% carboxymethylcellulose solution; group B: 400 mg/kg ornidazole (ORN); group C: 400 mg/kg+LC100 mg/kg. All rats were treated via gastric gavage for 20 d consecutively. After this treatment, the rats were killed and sperm density, motility ,the normal rate of sperm morphology and the concentration of L-carnitine in the epididymis were measured.Results: Compared with the normal control and C group, there were no obvious changes in sperm density (P 0. 05) , but a significant decrease in sperm motility and morphology in B group(P 0. 05) , and the concentration of L-carnitine obviously declined (P 0. 05). Epididymal sperm motility， sperm morphology and the total L-carnitine ， free L-carnitine concentration were positively correlated (r = 0.645, P 0.05; r = 0.676, P 0.05;r = 0.557, P 0.05; r = 0.583, P 0.05). There were statisti