高效液相色谱蒸发光散射检测法测定食品中果糖, 葡萄糖, 蔗糖, 乳糖和麦芽糖.PDFVIP

968 2007 6 17 6 Chinese Journal ofHea lth Laboratory echnology, Jun 2007; V ol 17 No 6 高效液相色谱蒸发光散射检测法测定食品中 果糖葡萄糖蔗糖乳糖和麦芽糖 蔡欣欣, 张秀尧 (, 325001) [] : M : , , C SPE, : ( 75: 25, v /v) , A lltech Prevail Carbohydrate 18 ES( 250! 46 mm, 5 m ) , : : 20 ~ 320 m g/L25~ 400 m g/L 25~ 400 m g/L, 4 m g/L5 mg /L 5 m g/L, 50~ 800 m g/L, 15 m g/L, 5 972% ~ 1064% , 056% ~ 21% : , [] 高效液相色谱; 激光蒸发光散射检测; 食品; 果糖; 葡萄糖; 蔗糖; 麦芽糖; 乳糖 + [ ] O6577 2 [] A [] 1004- 8685(2007) 06- 0968- 03 Analysis of fructose, glucose, sucrose, lactose and maltose in food by high performance liquid chromatographywith evaporative light scattering detector Ca i X inx in, Zhang X iuyao (W enzhou Center for Disease Control and Prevention,W enzhou 325001, Ch ina) [Abstract] Objective: o establish a smi p le, accurate and sensitivem ethod for determ ination of fructose, glucose, sucrose, lac tose and maltose in food by h igh perform ance liquid chrom atography w ith evaporative light scattering detectorMethods: he sug ars in food sam plesw ere extracted w ith w ater and the extracts w ere diluted w ith aceton itrile to rem ove aqueous soluble mi puri tiesFurther cleanupw as perform ed on a C cartridge to rem ove the nonpolar components he carbohydratesw ere separated on 18 M A lltech Prevail Carbohydrate ES column ( 250 ! 46 mm, 5 m) by using acetonitrile: w ater ( 75: 25, v/v) as the m obile phaseResults: he linear range w as 20~ 320 m g/L, 25~ 400 m g/L, 25~ 400 m g/L, 50~ 800 m g/L, 50~ 800 m g/L w ith the detection ijm