二藤通痹合剂抗类风湿有效部位药效学筛选.doc

二藤通痹合剂抗类风湿有效部位药效学筛选摘 要：目的：对二藤通痹合剂进行有效部位提取，并以抗类风湿药理作用验证其合理性，筛选出最优提取部位。方法：制作大鼠关节炎模型（AA模型），以各组提取液灌胃给药，测量大鼠足肿胀度，测定血清细胞因子白介素-2（IL-2）、白介素-6（IL-6），并取后肢关节做病理组织学检查。结果：二藤提取液Ⅰ、Ⅱ、Ⅲ组均对大鼠足肿胀有消炎消肿作用，其中Ⅱ组与模型组比较有统计学意义（P＜0.01）；二滕提取液Ⅱ、Ⅲ组有减轻关节滑膜病变、减轻关节软骨病变作用（P＜0.01）；二藤提取液Ⅰ、Ⅲ组能降低AA大鼠血中IL-2、IL-6水平。结论：优选组分Ⅲ为最佳提取液。? 关键词：二藤通痹合剂；大鼠关节炎模型；足肿胀度；白介素-2；白介素-6；病理检查? 中图分类号：R284.1文献标识码：A 文章编号：1673-7717(2008)11-2481-03? Pharmaceutical Screening of Effective Fraction of Erteng Tongbi Mixture? WANG Li?xin,REN Ming?neng,LI Xiao?bing,ZOU Yan?yan? (The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China)? Abstract:Objective:To select the best extractive fraction of Ertong Tongbi Mixtrue(ETM),the effective parts of ETM was extracted and thier rationality was proved by pharmacological tests on anti?theumatioid.Methods:The extractive was divided into three groups(Ⅰ,Ⅱ and Ⅲ).Rat arthritis model(AA modle)was made.The rat was infused by intragastric gavage with different groups of extractive,the degree of rat foot swelling was measured,the level of cytokine IL-2 and IL-6 in serum was tested,and the change of joints was checked by Pathological and histlolgy.Results:All extractive groups on foot swelling can mitigate inflammation and edema.The effects of group Ⅱ is gisnificantly compare to model group(P＜0.01).The effects of groups Ⅱ and Ⅲ on releasing synovial membrane arthropathy and cartilage injuring are remarkable(P＜0.01).The groups Ⅰ and Ⅲ can decrease the level of IL-2 and IL-6 in serum of AA rat.Conclusion:The best extractive is group Ⅲ.? Key words:ertong tongbi mixtrue;rat arthritis model;foot swelling;IL-2;IL-6;pathological examination 中药复方制剂二藤通痹合剂为本院注册制剂，临床用于治疗类风湿关节炎及强直性脊柱炎10多年，疗效显著[1-2]。该药起效快，具有显著的消肿、镇痛、改善关节功能等作用，病人服药5～6h即显效。在临床指标中，晨僵时间、休息痛、双手握力和压痛关节数均有显著的改善，在降低实验室指标方面，能显著降低病人的血沉(ＥＳＲ)、Ｃ反应蛋白(ＣＲＰ)和类风湿因子（ＲＦ）滴度。? 该方由中药南蛇藤、鸡血藤组成。南蛇藤（Celastrus orbiculatus Thunb）为卫矛科南蛇藤属植物，性温，味辛，用于祛风胜湿，行气散血，消肿解毒，治疗风湿筋骨疼痛、跌打损伤、痈疽肿毒等疾病。鸡血藤为豆科植物密花豆（S