前列腺素E1冻干隐形脂质体包封率测定.doc

前列腺素E1冻干隐形脂质体包封率测定摘要：目的评价前列腺素E1（PGE1）冻干隐形脂质体的质量，测定PGE1冻干隐形脂质体的包封率。方法用超滤法分离脂质体和游离药物；采用Hypersil C18柱（250 mm×4.6 mm，5 μm），流动相：pH 4.9的磷酸二氢钾溶液-乙腈（3∶2），流速1.0 mLmin-1，检测波长210 nm，测定药物含量，计算包封率。结果超滤法能很好的分离脂质体与游离药物，PGE1在1～50 mgL-1内线性关系良好（r=0.999 3），平均包封率为93.02 %。结论超滤-HPLC法测定PGE1冻干隐形脂质体包封率方法简便、准确。 关键词：超滤法；高效液相色谱法；前列腺素E1；隐形脂质体；包封率 中图分类号：R944.9文献标识码：A文章编号：1672-979X(2009)01-0042-03 Determination of Entrapment Efficiency of Freeze-dried PGE1 Stealth Liposomes SHI Nian-qiu1, ZHANG Da-tong 1, WANG Dong-kai2, GUAN Shi-xia 3, CHEN Yu-zhou4, CHEN Xiu-yi5 (1. College of Chemical Engineering, Shandong Institute of Light Industry, Jinan 250353, China; 2. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. Guangzhou Regex Biotech Co., Ltd, Guangzhou 510730, China; 4. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; 5. Shandong Institute of Pharmaceutical Industry, Jinan 250100, China) Abstract：Objective To evaluate the quality of freeze-dried prostaglandin E1(PGE1) stealth liposomes and determine the entrapment efficiency. Methods Liposomes and dissociative drug were separated by ultrafiltration. The content of PGE1 was determined by Hypersil C18 column (250 mm×4.6 mm，5 μm) with the mixture of KH2PO4(pH 4.9)-acetonitrile(3∶2) as mobile phase at the flow rate of 1.0 mL?min-1. The detection wavelength was 210 nm. The entrapment efficiency was calculated. Results It was good to separate liposomes and dissociative drug by ultrafiltration. There was a good linear relationship (r=0.999 3) when the concentration of PGE1 was 1～50 mg?L-1. The average entrapment efficiency was 93.02 %. Conclusion The ultrafiltration-HPLC method is convenient and accurate for the determination of entrapment efficiency of freeze-dried PGE1 stealth liposomes. Key words：ultrafiltration; HPLC; prostaglandin E1; stealth liposomes; entrapment efficiency 前列腺素E1（PGE1）是天然前列腺素（PG）类物质，20世纪30年代发现，60年代确定其化学结构为