双根清脑颗粒对VD模型大鼠行为学及脑微血管内皮细胞ICAM-1表达影响实验探究.doc

双根清脑颗粒对VD模型大鼠行为学及脑微血管内皮细胞ICAM-1表达影响实验探究摘 要:目的:观察双根清脑颗粒对VD模型大鼠行为学与脑微血管内皮细胞ICAM-1表达的影响,探讨双根清脑颗粒治疗VD的机理。方法:从颈外动脉插管缓慢注入同种血栓栓子造成VD大鼠模型。中药组以双根清脑颗粒干预,模型组、对照组、正常组给予生理盐水。Moriss水迷宫观察隐匿平台获得时间与大鼠空间搜索时间;免疫组织化学方法检测大鼠脑微血管ICAM-1的表达。结果:Moriss水迷宫试验发现,与模型组相比,中药组隐匿平台获得时间明显缩短(P0.05),大鼠空间搜索次数亦明显增多(P0.05)。免疫组织化学检测表明:与模型组相比,无论是1天,还是3天、7天、14天组,中药组均能有效降低VD模型大鼠脑微血管ICAM-1的表达(P0.01)。结论:双根清脑颗粒可改善VD模型大鼠的学习与记忆的功能,其机制与下调VD模型大鼠脑微血管内皮细胞ICAM-1表达有关。 关键词:血管性痴呆;双根清脑颗粒;行为学指标 ICAM-1 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0104-03 Laboratory Studies on the Effects of Shuanggen Cephalocathartic Granule on Model Ratswith Vascular Dementia in Behavioristics and the Expression of ICAM-1 on CMECs WU Hao?xin,LIU Xu?feng,ZHENG Hong?yan,CAI Jian?wei (Brain of Basic Researth of TCM Laboratory of Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China) Abstract:Objective:To observe the effects of shuanggen cephalocathartic granule on model rats with vascular dementia in behavioristics and the expressionofinter-cellular adhesion molecule-1( ICAM-1) on cerebral microvascular endothelial cells(CMECs).Methods:Blood embolus collected fromrat left ventriclewas injected into encenphalon from external carotid artery of the same rat to establish model of mulitiple cerebral infarction. Rats in Chinese herb group were given shuanggen cephalocathartic granule .Rats in model group, sham operation group and normal group were given physiological saline in equivalence. The time of gettinglatency of platform and the times of spatial exploring were observed by Morris water maze . Immunohistochemistry was used detecting the expression of ICAM-1 on CMECs. Results: Compared with model group, the time of getting latency of platform in Chinese herb group significantly shortened(P0.05), the times of spatial exploring in Chinese herb group significantly increased.(P0.05). shuanggen cephalocathartic granule could markedly reduce the expression of ICAM-1 on CMECs(P0.01). Conclusion:Shuanggen cephalocatha